Effect of flutianil on the morphology and gene expression of powdery mildew

Flutianil, a fungicide effective only on powdery mildew, was previously reported to affect the host cell's haustorial formation and nutrient absorption. Studies were conducted to investigate flutianil's primary site of action on Blumeria graminis morphology using transmission electron micr...

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Bibliographic Details
Published in:Journal of Pesticide Science 2021/05/20, Vol.46(2), pp.206-213
Main Authors: Kimura, Sachi, Shibata, Yusuke, Oi, Takao, Kawakita, Kazuhito, Takemoto, Daigo
Format: Article
Language:English
Subjects:
Airborne microorganisms
Blumeria graminis
Cell walls
flutianil
Fungi
Fungicides
Gene expression
Gene sequencing
Genes
Haustoria
haustorium
microscopy
Morphology
Organelles
Powdery mildew
Regular
Ribonucleic acid
RNA
RNA-seq
Transmission electron microscopy
ultrastructure
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Summary:Flutianil, a fungicide effective only on powdery mildew, was previously reported to affect the host cell's haustorial formation and nutrient absorption. Studies were conducted to investigate flutianil's primary site of action on Blumeria graminis morphology using transmission electron microscope (TEM) observation and RNA sequencing (RAN-seq) techniques. TEM observation revealed that flutianil caused the extra-haustorial matrix and fungal cell wall to be obscured, without remarkable changes of other fungal organelles. RNA-seq analysis indicated that, unlike other powdery-mildew fungicides, flutianil did not significantly affect the constantly expressed genes for the survival of B. graminis. Genes whose expression is up- or downregulated by flutianil were found; these are the three sugar transporter genes and various effector genes, mainly expressed in haustoria. These findings indicate that the primary site of action of flutianil might be in the haustoria.
ISSN:1348-589X
1349-0923
DOI:10.1584/jpestics.D21-003