Transcriptome analysis of primary chicken cells infected with infectious bronchitis virus strain K047-12 isolated in Korea

Infectious bronchitis virus (IBV), an avian coronavirus, is highly contagious. Chickens with IBV infection develop acute pathogenesis in multiple organs, including the respiratory and urogenital tracts. Frequent recombination in the spike (S) glycoprotein gene has made vaccine strategies ineffective...

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Bibliographic Details
Published in:Archives of virology 2021-08, Vol.166 (8), p.2291-2298
Main Authors: Lee, Rangyeon, Jung, Ji Seung, Yeo, Ji-in, Kwon, Hyuk Moo, Park, Jeongho
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Report
Bronchitis
Cells, Cultured
Chickens
Coronavirus Infections - virology
Coronaviruses
Cytokines
Cytokines - genetics
Gene expression
Gene Expression Profiling
Genetic distance
Host-Pathogen Interactions
Immune response
Immunity, Innate - genetics
Immunoregulation
Infectious bronchitis virus - classification
Infectious bronchitis virus - genetics
Infectious bronchitis virus - isolation & purification
Infectious bronchitis virus - physiology
Infectious Diseases
Innate immunity
Kidney - cytology
Medical Microbiology
Pathogenesis
Phylogeny
Recombination
Republic of Korea
Severe acute respiratory syndrome coronavirus 2
Spike Glycoprotein, Coronavirus - genetics
Transcriptomes
Vaccine development
Vaccines
Virology
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Summary:Infectious bronchitis virus (IBV), an avian coronavirus, is highly contagious. Chickens with IBV infection develop acute pathogenesis in multiple organs, including the respiratory and urogenital tracts. Frequent recombination in the spike (S) glycoprotein gene has made vaccine strategies ineffective. To understand IBV pathogenesis, we analyzed the genetic distance between Korean IBV isolates and other coronaviruses, including SARS-CoV-2. To obtain comprehensive information about early immune responses such as innate cytokine production and associated immune regulation during IBV infection, we infected primary chicken embryonic kidney cells and performed transcriptome analysis. We observed that the functional pathways of innate immunity are regulated and confirmed expression of genes that coordinate early immune responses. Understanding the immune profile of the host cell may assist in vaccine development.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-021-05124-9