The Effect of Anadara granosa Shell’s–Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing

• Published in: European journal of dentistry 2021-05, Vol.15 (2), p.228-235
• Main Authors: Sari, Rima Parwati, Revianti, Syamsulina, Andriani, Dwi, Prananingrum, Widyasri, Rahayu, Retno Pudji, Sudjarwo, Sri Agus
• Format: Article
• Language: English
• Subjects: Original; Original Article
• ISSN: 1305-7456; 1305-7464
• DOI: 10.1055/s-0040-1719215

Abstract Objectives  This article aimed to investigate the effect of Anadara granosa (AG) shell’s– Stichopus hermanni scaffold on cluster of differentiation (CD)44 and interleukin-10 (IL-10) expression to decrease osteoclasts in socket healing. Materials and Methods  Thirty male Wistar rats were divided into five groups. The lower left incisor was extracted, then given a placebo for group control (K), the treatment group was administered with scaffold from AG shells, and a treatment group with scaffold from blood cockle shell– S. hermanni with the concentration of 0.4, 0.8, and 1.6% (AGSH0.4; AGSH0.8; AGSH1.6). We made a bone graft from a combination of AGSH extract using the freeze-dried method. The socket was sutured by silk braid immediately. Third and Seventh days postextraction, animals are killed. CD44 and IL-10 expression were examined with immunohistochemistry, as well as osteoclast was examined with hematoxylin-eosin. Statistical Analysis  The data were analyzed using a one-way analysis of variance (for CD44 and osteoclast) and Kruskal–Wallis’ test (for IL-10) followed by a post hoc test in which the result of p < 0.05. Results  Scaffold from a combination of AGSH increased CD44 expression significantly, which enhanced IL-10 expression thereby decreased the number of osteoclasts in socket healing on days 3 and 7. Conclusion  Scaffold of AG shell– S. hermanni with a concentration of 0.8% was effective to enhance CD44 and IL-10 expression to decrease osteoclast in socket healing after tooth extraction.