Protective effects of recombinant 53-kDa protein of Trichinella spiralis on acute lung injury in mice via alleviating lung pyroptosis by promoting M2 macrophage polarization

• Published in: Innate immunity (London, England) England), 2021-05, Vol.27 (4), p.313-323
• Main Authors: Wei, Ling-yu, Jiang, An-qi, Jiang, Ren, Duan, Si-ying, Xu, Xue, Su, Ze-da-zhong, Xu, Jia
• Format: Article
• Language: English
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Acute Lung Injury - pathology; Alveoli; Animals; Arginase; Bronchoalveolar Lavage Fluid; Cell Count; Cell Polarity - drug effects; Cytokines - chemistry; Inflammatory diseases; Interleukin 10; Interleukin 13; Interleukin 4; Interleukins - antagonists & inhibitors; Lipopolysaccharides; Lungs; Macrophage Activation - drug effects; Macrophages; Macrophages - drug effects; Male; Mannose Receptor - metabolism; Mice; Mice, Inbred BALB C; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Original; Polarization; Protective Agents - pharmacology; Proteins; Pyroptosis; Pyroptosis - drug effects; Recombinant Proteins - pharmacology; Trichinella spiralis; Trichinella spiralis - chemistry
• ISSN: 1753-4259; 1753-4267
• DOI: 10.1177/17534259211013397

Trichinella spiralis represents an effective treatment for autoimmune and inflammatory diseases. The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl) was injected subcutaneously three times at an interval of 5 d before inducing ALI in the rTsP53+LPS group. Lung pathological score, the ratio and markers of classic activated macrophages (M1) and alternatively activated macrophages (M2), cytokine profiles in alveolar lavage fluid, and pyroptosis protein expression in lung tissue were investigated. RTsP53 decreased lung pathological score. Furthermore, rTsP53 suppressed inflammation by increasing IL-4, IL-10, and IL-13. There was an increase in alveolar M2 macrophage numbers, with an increase in CD206 and arginase-1-positive cells and a decrease in alveolar M1 markers such as CD197 and iNOS. In addition, the polarization of M2 macrophages induced by rTsP53 treatment could alleviate ALI by suppressing lung pyroptosis. RTsP53 was identified as a potential agent for treating LPS-induced ALI via alleviating lung pyroptosis by promoting M2 macrophage polarization.