Rapid multiplex PCR assays in patients with respiratory viral infections: is semi-quantitative data useful? A pilot study

Viral respiratory rapid multiplex PCR assays FilmArray® (FA) and ePlex® (eP) provide qualitative results which may not reflect clinical relevance. In a pilot study, we report retrospectively whether the semi-quantitative PCR assay R-GENE® would have facilitated clinical interpretation. Forty-four pa...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2021-09, Vol.52 (3), p.1173-1179
Main Authors: Gazeau, Pierre, Vallet, Sophie, Ansart, Séverine, Beauruelle, Clémence, Tran-Minoui, Adissa, Payan, Christopher, Pilorgé, Léa
Format: Article
Language:English
Subjects:
Assaying
Bacterial
Bacterial, Fungal and Virus Molecular Biology - Short Communication
Biomedical and Life Sciences
Exploitation
Food Microbiology
Fungal and Virus Molecular Biology - Short Communication
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Multiplexing
Mycology
Patients
Viruses
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Summary:Viral respiratory rapid multiplex PCR assays FilmArray® (FA) and ePlex® (eP) provide qualitative results which may not reflect clinical relevance. In a pilot study, we report retrospectively whether the semi-quantitative PCR assay R-GENE® would have facilitated clinical interpretation. Forty-four patients were hospitalized for various respiratory manifestations; all of them have benefited from a respiratory sample during acute symptoms. Among the 44 patients, FA detected 23 positive samples including 31 viruses, 26 of them gave high or moderate R-GENE® scores (cycle threshold 
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-021-00536-w