Functional and Structural Characterization of SARS-Cov-2 Spike Protein: An In Silico Study

• Published in: Ethiopian journal of health sciences 2021-03, Vol.31 (2), p.213-222
• Main Authors: Ebrahim-Saraie, Hadi Sedigh, Dehghani, Behzad, Mojtahedi, Ali, Shenagari, Mohammad, Hasannejad-Bibalan, Meysam
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 1029-1857; 1029-1857; 2413-7170
• DOI: 10.4314/ejhs.v31i2.2

BACKGROUND፡ Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the global outbreak of coronavirus disease 2019 (Covid-19), which has been considered as a pandemic by WHO. SARS-CoV-2 encodes four major structural proteins, among which spike protein has always been a main target for new vaccine studies. This in silico study aimed to investigate some physicochemical, functional, immunological, and structural features of spike protein using several bioinformatics tools.METHOD: We retrieved all SARS-CoV-2 spike protein sequences from different countries registered in NCBI GenBank. CLC Sequence Viewer was employed to translate and align the sequences, and several programs were utilized to predict B-cell epitopes. Modification sites such as phosphorylation, glycosylation, and disulfide bonds were defined. Secondary and tertiary structures of all sequences were further computed.RESULTS: Some mutations were determined, where only one (D614G) had a high prevalence. The mutations did not impact the B-cell and physicochemical properties of the spike protein. Seven disulfide bonds were specified and also predicted in several N-link glycosylation and phosphorylation sites. The results also indicated that spike protein is a non-allergen.CONCLUSION: In summary, our findings provided a deep understanding of spike protein, which can be valuable for future studies on SARS CoV-2 infections and design of new vaccines.