Taurine ameliorates thioacetamide induced liver fibrosis in rats via modulation of toll like receptor 4/nuclear factor kappa B signaling pathway

• Published in: Scientific reports 2021-06, Vol.11 (1), p.12296
• Main Authors: Younis, Nancy S, Ghanim, Amal M H, Elmorsy, Mohammad A, Metwaly, Heba A
• Format: Article
• Language: English
• Subjects: Actins - genetics; Animals; Antioxidants - pharmacology; Caspase 3 - genetics; Gene Expression Regulation - drug effects; Humans; Liver Cirrhosis - chemically induced; Liver Cirrhosis - drug therapy; Liver Cirrhosis - genetics; Liver Cirrhosis - pathology; Lymphocyte Antigen 96 - genetics; Molecular Docking Simulation; NF-kappa B - genetics; Protein Binding - drug effects; Rats; Serum Albumin - drug effects; Signal Transduction - drug effects; Taurine - genetics; Taurine - pharmacology; Thioacetamide - toxicity; Toll-Like Receptor 4 - genetics
• ISSN: 2045-2322
• DOI: 10.1038/s41598-021-91666-6

Liver fibrosis is a significant health problem that can cause serious illness and death. Unfortunately, a standard treatment for liver fibrosis has not been approved yet due to its complicated pathogenesis. The current study aimed at assessing the anti-fibrotic effect of taurine against thioacetamide induced liver fibrosis in rats through the modulation of toll like receptor 4/nuclear factor kappa B signaling pathway. Both concomitant and late taurine treatment (100 mg/kg, IP, daily) significantly reduced the rise in serum ALT and AST activities and significantly reversed the decrease in serum albumin and total protein. These results were confirmed by histopathological examinations and immunehistochemical inspection of α-SMA, caspase-3 and NF-κB. The antioxidant potential of taurine was verified by a marked increase of GSH content and a reduction of MDA level in liver tissue. The anti-fibrotic effects of taurine were evaluated by investigating the expression of TLR4, NF-κB. The protein levels of IL-6, LPS, MyD88, MD2, CD14, TGF-β1 and TNF-α were determined. Docking studies were carried out to understand how taurine interacts inside TLR4-MD2 complex and it showed good binding with the hydrophobic binding site of MD2. We concluded that the anti-fibrotic effect of taurine was attributable to the modulation of the TLR4/NF-κB signaling.