Physiological Characterization of Sulfolobus acidocaldarius in a Controlled Bioreactor Environment

The crenarchaeal model organism Sulfolobus acidocaldarius is typically cultivated in shake flasks. Although shake flasks represent the state-of-the-art for the cultivation of this microorganism, in these systems crucial process parameters, like pH or substrate availability, are only set initially, b...

Full description

Saved in:
Bibliographic Details
Published in:International journal of environmental research and public health 2021-05, Vol.18 (11), p.5532
Main Authors: Rastädter, Kerstin, Wurm, David Johannes, Spadiut, Oliver, Quehenberger, Julian
Format: Article
Language:English
Subjects:
Biomass
Bioreactors
Carbon
Carbon sources
Cell culture
Chromatography
Controlled conditions
Cultivation
Dilution
Flasks
Glucose
Glutamic acid
Growth conditions
Metabolites
Microorganisms
Physiology
Process controls
Process parameters
Sulfolobus acidocaldarius
Trehalose
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The crenarchaeal model organism Sulfolobus acidocaldarius is typically cultivated in shake flasks. Although shake flasks represent the state-of-the-art for the cultivation of this microorganism, in these systems crucial process parameters, like pH or substrate availability, are only set initially, but cannot be controlled during the cultivation process. As a result, a thorough characterization of growth parameters under controlled conditions is still missing for S. acidocaldarius. In this study, we conducted chemostat cultivations at 75 °C using a growth medium containing L-glutamate and D-glucose as main carbon sources. Different pH values and dilution rates were applied with the goal to physiologically characterize the organism in a controlled bioreactor environment. Under these controlled conditions a pH optimum of 3.0 was determined. Washout of the cells occurred at a dilution rate of 0.097 h−1 and the optimal productivity of biomass was observed at a dilution rate of 0.062 h−1. While both carbon sources were taken up by S. acidocaldarius concomitantly, a 6.6-fold higher affinity for L-glutamate was shown. When exposed to suboptimal growth conditions, S. acidocaldarius reacted with a change in the respiratory behavior and an increased trehalose production rate in addition to a decreased growth rate.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph18115532