Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr

The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid- beta 1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay,...

Full description

Saved in:
Bibliographic Details
Published in:3 Biotech 2021-07, Vol.11 (7), p.330, Article 330
Main Authors: Tan, Mario A., Tan, Byron Leander U., Nonato, Maribel G., An, Seong Soo A.
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Cell viability
Chemistry
Chemistry and Materials Science
Cytotoxicity
Membrane potential
Mitochondria
Neuroblastoma
Neuroprotection
Pandanus
Phytosterols
Placoneis clementis
Reactive oxygen species
Short Reports
Stem Cells
Toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid- beta 1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and in vitro neuroprotective cell viability, intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were evaluated with human neuroblastoma SH-SY5Y cells insulted with Aβ. Chromatographic separation on the alcoholic extract yielded known phytosterols. Results showed that pretreatment of the SH-SY5Y cells with the P. clementis extracts increased cell viability and MMP, and decreased ROS, suggesting protective effects. Hence, P. clementis extract has promising neuroprotective therapeutic potential.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-021-02889-3