Transmission Dynamics and Microevolution of Neisseria meningitidis During Carriage and Invasive Disease in High School Students in Georgia and Maryland, 2006–2007

• Published in: The Journal of infectious diseases 2021-06, Vol.223 (12), p.2038-2047
• Main Authors: Mustapha, Mustapha M, Marsh, Jane W, Shutt, Kathleen A, Schlackman, Jessica, Ezeonwuka, Chinelo, Farley, Monica M, Stephens, David S, Wang, Xin, Van Tyne, Daria, Harrison, Lee H
• Format: Article
• Language: English
• Subjects: Adolescent; Bacterial infections; Carrier State - epidemiology; Disease transmission; Fimbriae Proteins - genetics; Gene mapping; Genomes; Georgia - epidemiology; Humans; Major and Brief Reports; Maryland - epidemiology; Meningitis; Meningococcal Infections - epidemiology; Meningococcal Infections - transmission; Multilocus sequence typing; Neisseria meningitidis; Neisseria meningitidis - genetics; Nucleotide sequence; Pediatrics; Pharynx; Pilin; Schools; Secondary school students; Students; Whole genome sequencing
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/jiaa674

Abstract Background The mechanisms by which Neisseria meningitidis cause persistent human carriage and transition from carriage to invasive disease have not been fully elucidated. Methods Georgia and Maryland high school students were sampled for pharyngeal carriage of N. meningitidis during the 2006–2007 school year. A total of 321 isolates from 188 carriers and all 67 invasive disease isolates collected during the same time and from the same geographic region underwent whole-genome sequencing. Core-genome multilocus sequence typing was used to compare allelic profiles, and direct read mapping was used to study strain evolution. Results Among 188 N. meningitidis culture–positive students, 98 (52.1%) were N. meningitidis culture positive at 2 or 3 samplings. Most students who were positive at >1 sampling (98%) had persistence of a single strain. More than a third of students carried isolates that were highly genetically related to isolates from other students in the same school, and occasional transmission within the same county was also evident. The major pilin subunit gene, pilE, was the most variable gene, and no carrier had identical pilE sequences at different time points. Conclusion We found strong evidence of local meningococcal transmission at both the school and county levels. Allelic variation within genes encoding bacterial surface structures, particularly pilE, was common. Neisseria meningitidis is often carried asymptomatically. We found frequent transmission of meningococci among students in the same school, and occasional transmission within the same county. Carried meningococci show very high rate of recombination within genes encoding type 4 pili.