Molecular Characterization of China Human Rabies Vaccine Strains

To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally consider...

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Bibliographic Details
Published in:Virologica Sinica 2013-04, Vol.28 (2), p.116-123
Main Authors: Tao, Xiaoyan, Han, Na, Guo, Zhenyang, Tang, Qing, Rayner, Simon, Liang, Guodong
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
China
crystal structure
GenBank
genome
Genome, Viral - genetics
Glycoproteins - genetics
Glycoproteins - immunology
Glycoproteins - metabolism
Humans
Lyssavirus
Medical Microbiology
Microbial Genetics and Genomics
Microbiology
mutation
Oncology
rabies
Rabies Vaccines - immunology
Rabies virus - immunology
Rabies virus - metabolism
Research Article
topology
Vesicular stomatitis virus
Vesiculovirus
Viral Proteins - genetics
Viral Proteins - immunology
Viral Proteins - metabolism
Virology
virulence
viruses
中国人
分子特征
水泡性口炎病毒
狂犬病
疫苗株
病毒基因组
胞外结构域
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Summary:To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to this end we predicted the tertiary structure of the G protein of aG strain, CTN181 strain and wild type strain HN10 based on the crystal structure of Vesicular stomatitis virus (VSV) G. The predicted RABV G structure has a similar topology to VSV G and the ectodomain can be divided into 4 distinct domains DI — DIV. By mapping the characterized mutations to this structure between China vaccine strains and their close street strains, we speculate that the G303(P-H) mutations of CTN181 and HN10 causing DII 3D change may be associated with the II attenuated virulence in both strains. Specifically, the two signature mutations (G165P and G231P) in the aG strain are withinßsheets, suggesting that both sites are of structural importance.
ISSN:1674-0769
1995-820X
DOI:10.1007/s12250-013-3314-9