Parallel bioreactor system for accessible and reproducible anaerobic culture

• Published in: Access microbiology 2021-04, Vol.3 (4), p.000225-000225
• Main Authors: Monaghan, Taylor I., Baker, Joseph A., Robinson, Gary K., Shepherd, Mark
• Format: Article
• Language: English
• Subjects: Methods
• ISSN: 2516-8290; 2516-8290
• DOI: 10.1099/acmi.0.000225

When working with anaerobic bacteria it is important to have the capability to perform parallel bioreactor growth experiments that are both controllable and reproducible, although capital and consumables costs for commercially available systems are often prohibitively high. Hence, a three-vessel parallel bioreactor system was designed and constructed that has the capabilities for batch and fed batch processes and can also be set up for continuous culture at a fraction of the cost of commercial systems. This system carries over many of the same functionalities of those systems with a higher price point of entry, including in-line monitoring of temperature, pH, and redox poise. To validate the performance of this system Clostridium saccharoperbutylacetonicum was grown under conditions that promote ABE fermentation, an established industrial process used to produce the solvents acetone, butanol and ethanol. Measurements of cell density, pH, and redox poise all confirmed reproducible culture conditions for these parallel vessels, and solvent quantitation via GCMS verified consistent metabolic activities for the separate cultures. In future, this system will be of interest to researchers that require high performance parallel fermentation platforms but where commercial systems are not accessible.