Metabolipidomic profiling reveals an age‐related deficiency of skeletal muscle pro‐resolving mediators that contributes to maladaptive tissue remodeling

• Published in: Aging cell 2021-06, Vol.20 (6), p.e13393-n/a
• Main Authors: Markworth, James F., Brown, Lemuel A., Lim, Eunice, Castor‐Macias, Jesus A., Larouche, Jacqueline, Macpherson, Peter C. D., Davis, Carol, Aguilar, Carlos A., Maddipati, Krishna Rao, Brooks, Susan V.
• Format: Article
• Language: English
• Subjects: Acids; Age; Aging; Aging - physiology; Animals; Biosynthesis; cellular immunology; Chromatography; Cytochrome P450; Cytokines; Docosahexaenoic acid; Eicosapentaenoic acid; Epoxides; Fibrosis; Genotype & phenotype; Geriatrics; Humans; Inflammation; Inflammation - metabolism; injury; Lipids; Lipoxin A4; Lipoxygenase; Liquid chromatography; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; Metabolism - physiology; Metabolites; Mice; Muscle function; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Neutrophils; Original; Physiological aspects; Principal components analysis; Prostaglandin E2; Prostaglandin endoperoxide synthase; sarcopenia; satellite stem cell; Scientific imaging; Skeletal muscle; Tissue engineering; Tissue Engineering - methods
• ISSN: 1474-9718; 1474-9726
• DOI: 10.1111/acel.13393

Specialized pro‐resolving mediators actively limit inflammation and support tissue regeneration, but their role in age‐related muscle dysfunction has not been explored. We profiled the mediator lipidome of aging muscle via liquid chromatography‐tandem mass spectrometry and tested whether treatment with the pro‐resolving mediator resolvin D1 (RvD1) could rejuvenate the regenerative ability of aged muscle. Aged mice displayed chronic muscle inflammation and this was associated with a basal deficiency of pro‐resolving mediators 8‐oxo‐RvD1, resolvin E3, and maresin 1, as well as many anti‐inflammatory cytochrome P450‐derived lipid epoxides. Following muscle injury, young and aged mice produced similar amounts of most pro‐inflammatory eicosanoid metabolites of cyclooxygenase (e.g., prostaglandin E2) and 12‐lipoxygenase (e.g., 12‐hydroxy‐eicosatetraenoic acid), but aged mice produced fewer markers of pro‐resolving mediators including the lipoxins (15‐hydroxy‐eicosatetraenoic acid), D‐resolvins/protectins (17‐hydroxy‐docosahexaenoic acid), E‐resolvins (18‐hydroxy‐eicosapentaenoic acid), and maresins (14‐hydroxy‐docosahexaenoic acid). Similar absences of downstream pro‐resolving mediators including lipoxin A4, resolvin D6, protectin D1/DX, and maresin 1 in aged muscle were associated with greater inflammation, impaired myofiber regeneration, and delayed recovery of strength. Daily intraperitoneal injection of RvD1 had minimal impact on intramuscular leukocyte infiltration and myofiber regeneration but suppressed inflammatory cytokine expression, limited fibrosis, and improved recovery of muscle function. We conclude that aging results in deficient local biosynthesis of specialized pro‐resolving mediators in muscle and that immunoresolvents may be attractive novel therapeutics for the treatment of muscular injuries and associated pain in the elderly, due to positive effects on recovery of muscle function without the negative side effects on tissue regeneration of non‐steroidal anti‐inflammatory drugs. Chronic low‐grade inflammation of aging muscle was associated with a basal deficiency of maresin 1, resolvin E3, 8‐oxo‐resolvin D1, and anti‐inflammatory fatty acid epoxides. Aged mice produced normal amounts of most prostaglandins following muscle injury but were deficient in local biosynthesis of markers of the lipoxins, E‐resolvins, D‐resolvins, and maresins. Systemic treatment with resolvin D1 suppressed inflammatory cytokine expression, limited muscle fibrosis, a