Molecular docking and statistical optimization of taurocholate-stabilized galactose anchored bilosomes for the enhancement of sofosbuvir absorption and hepatic relative targeting efficiency

• Published in: Drug delivery 2020-01, Vol.27 (1), p.996-1009
• Main Authors: Joseph Naguib, Marianne, Moustafa Kamel, Amira, Thabet Negmeldin, Ahmed, Elshafeey, Ahmed Hassen, Elsayed, Ibrahim
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Antiviral Agents - administration & dosage; Antiviral Agents - pharmacokinetics; Antiviral Agents - pharmacology; Antiviral drugs; Bile; bile salts; bilosomes; Chemistry, Pharmaceutical - methods; Drug Carriers - chemistry; Drug dosages; Drug Stability; Efficiency; Freeze Drying; galactose; Galactose - chemistry; Hepatitis; Hexoses - chemistry; Liposomes - chemistry; Liver; liver targeting; Mice; Mice, Inbred BALB C; molecular docking; Molecular Docking Simulation; Nanoparticles - chemistry; Oral administration; Particle Size; Permeability; Pharmaceutical sciences; Pharmacy; Sofosbuvir; Sofosbuvir - administration & dosage; Sofosbuvir - pharmacokinetics; Sofosbuvir - pharmacology; taurocholate; Taurocholic Acid - chemistry
• ISSN: 1071-7544; 1521-0464
• DOI: 10.1080/10717544.2020.1787557

The work aimed to improve both absorption and hepatic availability of sofosbuvir. Bilosomes and galactose-anchored bilosomes were investigated as potential nanocarriers for this purpose. Sofosbuvir is a class III drug with high solubility and low permeability. Thus, the drug entrapment into lipid-based galactose-anchored carriers would enhance drug permeability and improve its liver availability. The galactosylated taurocholate was designed and synthesized based on molecular docking studies, where both galactose and taurocholate molecules were connected in a way to avoid affecting crucial interactions and avoid steric clashes with their cellular uptake receptors. The suggested nano-carriers were prepared using a thin-film hydration technique with sodium taurocholate and span 60 as stabilizers. The prepared formulae were statistically optimized using a central composite design. The optimized plain and galactosylated formulae, composed of SAA to drug ratio of 1:1 w/w and sodium taurocholate to span ratio of 10:1 w/w, have a vesicular size, zeta potential and entrapment efficiency in the range of 140-150 nm, −50 mV and 85%, respectively. The optimized formulae were lyophilized to increase their physical stability and facilitate accurate drug dosing. In vivo results showed that Sofosbuvir availability in the liver was significantly increased after oral administration of the plain and the galactosylated bilosomal formulae when compared to the oral drug solution with relative targeting efficiencies (RTIs) of 1.51 and 3.66, respectively. These findings confirmed the hypothesis of considering the galactosylated bilosomes a promising nanocarrier to efficiently target sofosbuvir to the liver.