Loading…
Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants
promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDR a widely used assay evaluating this region, wrongly classified 17/410 isolates as promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and t...
Saved in:
| Published in: | Antimicrobial agents and chemotherapy 2021-06, Vol.65 (7), p.e0250220-e0250220 |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-a375t-af811de25951c4ee3927236b2584fb8b9e78e2e3c1d44c0c0410719cf352874b3 |
| container_end_page | e0250220 |
| container_issue | 7 |
| container_start_page | e0250220 |
| container_title | Antimicrobial agents and chemotherapy |
| container_volume | 65 |
| creator | Ley, Serej D Pillay, Samantha Streicher, Elizabeth M van der Heijden, Yuri F Sirgel, Frederik Derendinger, Brigitta de Kock, Marianna Gagneux, Sébastien Warren, Robin M Theron, Grant de Vos, Margaretha |
| description | promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDR
a widely used assay evaluating this region, wrongly classified 17/410 isolates as
promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel
promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDR
can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing. |
| doi_str_mv | 10.1128/AAC.02502-20 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8218670</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2518989103</sourcerecordid><originalsourceid>FETCH-LOGICAL-a375t-af811de25951c4ee3927236b2584fb8b9e78e2e3c1d44c0c0410719cf352874b3</originalsourceid><addsrcrecordid>eNp1kU1P3DAQhq2qVdnS3nqufCxSA_6IE7sHpNX2CxVohYCr5TiTxTSxt7bTav8Cv5rAAoIDp9FoXj0z77wIvadkl1Im9-bzxS5hgrCCkRdoRomSRSVU9RLNCKmqopSk3EJvUrokUy8UeY22OFeEU8pn6OoI-uz8EucLwOAS_oyPg28hg80ueBw6_NN4M6yt8_gEkkvZeAv4yMQ_EBNu1vgkjBMB8Bdnlj6k7Cw-hZQTNr7FBy347DpnzT3uGP7fLvodwxAyRHxuojM-p7foVWf6BO_u6jY6-_b1dPGjOPz1_WAxPywMr0UuTCcpbYEJJagtAbhiNeNVw4Qsu0Y2CmoJDLilbVlaYklJSU2V7bhgsi4bvo32N9zV2AzQ2unAaHq9im4wca2DcfrpxLsLvQz_tGRUVjWZAB_vADH8HSerenDJQt8bD2FMmgkqlVSU8En6aSO1MaQUoXtYQ4m-iU9P8enb-DS7Ie9s5CYNTF-GMfrpE89pPzy28QC-z5ZfA_tho-w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2518989103</pqid></control><display><type>article</type><title>Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants</title><source>American Society for Microbiology Journals</source><source>PubMed Central</source><creator>Ley, Serej D ; Pillay, Samantha ; Streicher, Elizabeth M ; van der Heijden, Yuri F ; Sirgel, Frederik ; Derendinger, Brigitta ; de Kock, Marianna ; Gagneux, Sébastien ; Warren, Robin M ; Theron, Grant ; de Vos, Margaretha</creator><creatorcontrib>Ley, Serej D ; Pillay, Samantha ; Streicher, Elizabeth M ; van der Heijden, Yuri F ; Sirgel, Frederik ; Derendinger, Brigitta ; de Kock, Marianna ; Gagneux, Sébastien ; Warren, Robin M ; Theron, Grant ; de Vos, Margaretha</creatorcontrib><description>promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDR
a widely used assay evaluating this region, wrongly classified 17/410 isolates as
promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel
promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDR
can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing.</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AAC.02502-20</identifier><identifier>PMID: 33903113</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Antitubercular Agents - pharmacology ; Antitubercular Agents - therapeutic use ; Diagnostic Tests, Routine ; Drug Resistance, Multiple, Bacterial ; Genotype ; Humans ; Kanamycin - pharmacology ; Kanamycin Resistance - genetics ; Mechanisms of Resistance ; Microbial Sensitivity Tests ; Mutation - genetics ; Mycobacterium tuberculosis - genetics ; Tuberculosis, Multidrug-Resistant - diagnosis ; Tuberculosis, Multidrug-Resistant - drug therapy ; Tuberculosis, Multidrug-Resistant - genetics</subject><ispartof>Antimicrobial agents and chemotherapy, 2021-06, Vol.65 (7), p.e0250220-e0250220</ispartof><rights>Copyright © 2021 American Society for Microbiology.</rights><rights>Copyright © 2021 American Society for Microbiology. 2021 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a375t-af811de25951c4ee3927236b2584fb8b9e78e2e3c1d44c0c0410719cf352874b3</cites><orcidid>0000-0003-3059-2986 ; 0000-0002-2705-9120 ; 0000-0001-7783-9048</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.asm.org/doi/pdf/10.1128/AAC.02502-20$$EPDF$$P50$$Gasm2$$H</linktopdf><linktohtml>$$Uhttps://journals.asm.org/doi/full/10.1128/AAC.02502-20$$EHTML$$P50$$Gasm2$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,52751,52752,52753,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33903113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ley, Serej D</creatorcontrib><creatorcontrib>Pillay, Samantha</creatorcontrib><creatorcontrib>Streicher, Elizabeth M</creatorcontrib><creatorcontrib>van der Heijden, Yuri F</creatorcontrib><creatorcontrib>Sirgel, Frederik</creatorcontrib><creatorcontrib>Derendinger, Brigitta</creatorcontrib><creatorcontrib>de Kock, Marianna</creatorcontrib><creatorcontrib>Gagneux, Sébastien</creatorcontrib><creatorcontrib>Warren, Robin M</creatorcontrib><creatorcontrib>Theron, Grant</creatorcontrib><creatorcontrib>de Vos, Margaretha</creatorcontrib><title>Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDR
a widely used assay evaluating this region, wrongly classified 17/410 isolates as
promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel
promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDR
can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing.</description><subject>Antitubercular Agents - pharmacology</subject><subject>Antitubercular Agents - therapeutic use</subject><subject>Diagnostic Tests, Routine</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Genotype</subject><subject>Humans</subject><subject>Kanamycin - pharmacology</subject><subject>Kanamycin Resistance - genetics</subject><subject>Mechanisms of Resistance</subject><subject>Microbial Sensitivity Tests</subject><subject>Mutation - genetics</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Tuberculosis, Multidrug-Resistant - diagnosis</subject><subject>Tuberculosis, Multidrug-Resistant - drug therapy</subject><subject>Tuberculosis, Multidrug-Resistant - genetics</subject><issn>0066-4804</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kU1P3DAQhq2qVdnS3nqufCxSA_6IE7sHpNX2CxVohYCr5TiTxTSxt7bTav8Cv5rAAoIDp9FoXj0z77wIvadkl1Im9-bzxS5hgrCCkRdoRomSRSVU9RLNCKmqopSk3EJvUrokUy8UeY22OFeEU8pn6OoI-uz8EucLwOAS_oyPg28hg80ueBw6_NN4M6yt8_gEkkvZeAv4yMQ_EBNu1vgkjBMB8Bdnlj6k7Cw-hZQTNr7FBy347DpnzT3uGP7fLvodwxAyRHxuojM-p7foVWf6BO_u6jY6-_b1dPGjOPz1_WAxPywMr0UuTCcpbYEJJagtAbhiNeNVw4Qsu0Y2CmoJDLilbVlaYklJSU2V7bhgsi4bvo32N9zV2AzQ2unAaHq9im4wca2DcfrpxLsLvQz_tGRUVjWZAB_vADH8HSerenDJQt8bD2FMmgkqlVSU8En6aSO1MaQUoXtYQ4m-iU9P8enb-DS7Ie9s5CYNTF-GMfrpE89pPzy28QC-z5ZfA_tho-w</recordid><startdate>20210617</startdate><enddate>20210617</enddate><creator>Ley, Serej D</creator><creator>Pillay, Samantha</creator><creator>Streicher, Elizabeth M</creator><creator>van der Heijden, Yuri F</creator><creator>Sirgel, Frederik</creator><creator>Derendinger, Brigitta</creator><creator>de Kock, Marianna</creator><creator>Gagneux, Sébastien</creator><creator>Warren, Robin M</creator><creator>Theron, Grant</creator><creator>de Vos, Margaretha</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3059-2986</orcidid><orcidid>https://orcid.org/0000-0002-2705-9120</orcidid><orcidid>https://orcid.org/0000-0001-7783-9048</orcidid></search><sort><creationdate>20210617</creationdate><title>Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants</title><author>Ley, Serej D ; Pillay, Samantha ; Streicher, Elizabeth M ; van der Heijden, Yuri F ; Sirgel, Frederik ; Derendinger, Brigitta ; de Kock, Marianna ; Gagneux, Sébastien ; Warren, Robin M ; Theron, Grant ; de Vos, Margaretha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a375t-af811de25951c4ee3927236b2584fb8b9e78e2e3c1d44c0c0410719cf352874b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antitubercular Agents - pharmacology</topic><topic>Antitubercular Agents - therapeutic use</topic><topic>Diagnostic Tests, Routine</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Genotype</topic><topic>Humans</topic><topic>Kanamycin - pharmacology</topic><topic>Kanamycin Resistance - genetics</topic><topic>Mechanisms of Resistance</topic><topic>Microbial Sensitivity Tests</topic><topic>Mutation - genetics</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Tuberculosis, Multidrug-Resistant - diagnosis</topic><topic>Tuberculosis, Multidrug-Resistant - drug therapy</topic><topic>Tuberculosis, Multidrug-Resistant - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ley, Serej D</creatorcontrib><creatorcontrib>Pillay, Samantha</creatorcontrib><creatorcontrib>Streicher, Elizabeth M</creatorcontrib><creatorcontrib>van der Heijden, Yuri F</creatorcontrib><creatorcontrib>Sirgel, Frederik</creatorcontrib><creatorcontrib>Derendinger, Brigitta</creatorcontrib><creatorcontrib>de Kock, Marianna</creatorcontrib><creatorcontrib>Gagneux, Sébastien</creatorcontrib><creatorcontrib>Warren, Robin M</creatorcontrib><creatorcontrib>Theron, Grant</creatorcontrib><creatorcontrib>de Vos, Margaretha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ley, Serej D</au><au>Pillay, Samantha</au><au>Streicher, Elizabeth M</au><au>van der Heijden, Yuri F</au><au>Sirgel, Frederik</au><au>Derendinger, Brigitta</au><au>de Kock, Marianna</au><au>Gagneux, Sébastien</au><au>Warren, Robin M</au><au>Theron, Grant</au><au>de Vos, Margaretha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2021-06-17</date><risdate>2021</risdate><volume>65</volume><issue>7</issue><spage>e0250220</spage><epage>e0250220</epage><pages>e0250220-e0250220</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>promoter mutations can confer reduced Mycobacterium tuberculosis kanamycin susceptibility. GenoType MTBDR
a widely used assay evaluating this region, wrongly classified 17/410 isolates as
promoter wild type. Six out of seventeen isolates harbored mutations known to confer kanamycin resistance, and the remainder harbored either novel
promoter mutations (7/11) or disputed mutations (4/11). GenoType MTBDR
can miss established and new variants that cause reduced susceptibility. These data highlight the importance of reflex phenotypic kanamycin testing.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>33903113</pmid><doi>10.1128/AAC.02502-20</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3059-2986</orcidid><orcidid>https://orcid.org/0000-0002-2705-9120</orcidid><orcidid>https://orcid.org/0000-0001-7783-9048</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0066-4804 |
| ispartof | Antimicrobial agents and chemotherapy, 2021-06, Vol.65 (7), p.e0250220-e0250220 |
| issn | 0066-4804 1098-6596 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8218670 |
| source | American Society for Microbiology Journals; PubMed Central |
| subjects | Antitubercular Agents - pharmacology Antitubercular Agents - therapeutic use Diagnostic Tests, Routine Drug Resistance, Multiple, Bacterial Genotype Humans Kanamycin - pharmacology Kanamycin Resistance - genetics Mechanisms of Resistance Microbial Sensitivity Tests Mutation - genetics Mycobacterium tuberculosis - genetics Tuberculosis, Multidrug-Resistant - diagnosis Tuberculosis, Multidrug-Resistant - drug therapy Tuberculosis, Multidrug-Resistant - genetics |
| title | Melting the eis : Nondetection of Kanamycin Resistance Markers by Routine Diagnostic Tests and Identification of New eis Promoter Variants |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T07%3A50%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Melting%20the%20eis%20:%20Nondetection%20of%20Kanamycin%20Resistance%20Markers%20by%20Routine%20Diagnostic%20Tests%20and%20Identification%20of%20New%20eis%20Promoter%20Variants&rft.jtitle=Antimicrobial%20agents%20and%20chemotherapy&rft.au=Ley,%20Serej%20D&rft.date=2021-06-17&rft.volume=65&rft.issue=7&rft.spage=e0250220&rft.epage=e0250220&rft.pages=e0250220-e0250220&rft.issn=0066-4804&rft.eissn=1098-6596&rft_id=info:doi/10.1128/AAC.02502-20&rft_dat=%3Cproquest_pubme%3E2518989103%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a375t-af811de25951c4ee3927236b2584fb8b9e78e2e3c1d44c0c0410719cf352874b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2518989103&rft_id=info:pmid/33903113&rfr_iscdi=true |