High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications

In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRN...

Full description

Saved in:
Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2020-03, Vol.22 (12), p.6492-6506
Main Authors: Blanco, Celia, Verbanic, Samuel, Seelig, Burckhard, Chen, Irene A
Format: Article
Language:English
Subjects:
Chemists
Data analysis
Experiments
Gene Expression Profiling
Gene sequencing
High-Throughput Nucleotide Sequencing - standards
High-Throughput Nucleotide Sequencing - trends
Impact analysis
In Vitro Techniques
Peptides
RNA, Messenger - chemistry
Sequence Analysis, Protein - instrumentation
Sequence Analysis, Protein - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRNA display selections. In this Perspective, we introduce aspects of mRNA display and HTS that may be of interest to physical chemists. We highlight the potential of HTS to analyze in vitro selections of peptides and review recent advances in the application of HTS analysis to mRNA display experiments. We discuss some possible issues involved with HTS analysis and summarize some strategies to alleviate them. Finally, the potential for future impact of advancing HTS analysis on mRNA display experiments is discussed.
ISSN:1463-9076
1463-9084
DOI:10.1039/c9cp05912a