The Mechanism of the Yigutang-Mediated P13K/AKT/GSK-3β Signal Pathway to Regulate Osteogenic Differentiation of Bone Marrow Stromal Stem Cells to Treat Osteoporosis

Objectives. To explore the mechanism of Yigutang mediating the P13K/AKT/GSK-3β signaling pathway to regulate the osteogenic differentiation of bone marrow stromal stem cells to treat osteoporosis (OP). Methods. Sixty 12-week-old female SD rats were randomly divided into the normal group, model group...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2021, Vol.2021, p.6699781-8
Main Authors: Li, Ning, Gong, Yichen
Format: Article
Language:English
Subjects:
AKT protein
Alternative medicine
Antibodies
Bone marrow
Bone mineral density
Chinese medicine
Drugs
Estrogens
Femur
Fractures
Gene expression
Labeling
Metaphysis
Osteoblastogenesis
Osteoblasts
Osteoporosis
Ovaries
Rodents
Signal transduction
Stem cell transplantation
Stem cells
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Summary:Objectives. To explore the mechanism of Yigutang mediating the P13K/AKT/GSK-3β signaling pathway to regulate the osteogenic differentiation of bone marrow stromal stem cells to treat osteoporosis (OP). Methods. Sixty 12-week-old female SD rats were randomly divided into the normal group, model group, Yigutang group, and estrogen group, with 15 cases in each group. In the model group, Yigutang group, and estrogen group, the ovaries on both sides were removed to construct the model, and the bone mineral density (BMD) of the upper metaphysis of the right femur of the rats in each group was detected. The left femur of each group of rats was removed, and the load-deformation curve of the left femur of each group of rats was calculated. The number of osteoblasts was observed by H&E staining. After extracting the right femurs of rats in each group, real-time fluorescent quantitative PCR and Western blot were used to detect the expression levels of genes and proteins related to the P13K/AKT/GSK-3β signaling pathway. Results. The BMD of the upper metaphysis of the right femur in the Yigutang group and the estrogen group was significantly higher than that of the model group (P0.05). In addition, there was no significant difference between the Yigutang group and the estrogen group (P>0.05). The elastic load and maximum load of the Yigutang group and the estrogen group were significantly higher than the model group (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2021/6699781