A single-cell–resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle

A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of desce...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2021-06, Vol.118 (25), p.1-9
Main Authors: Yang, Yun, Wang, Hao, He, Jia, Shi, Wenchao, Jiang, Zhanmei, Gao, Lina, Jiang, Yan, Ni, Rui, Yang, Qifen, Luo, Lingfei
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cell Cycle
Cell fate
Cell Lineage
Cell Proliferation
Cells (biology)
Developmental biology
Embryogenesis
Embryonic growth stage
Endoderm
Endoderm - cytology
Foregut
G1 Phase
Hedgehog Proteins - metabolism
Pancreas
Pancreas - cytology
Population studies
Populations
Progenitor cells
Signal Transduction
Single-Cell Analysis
Stem cells
Stem Cells - cytology
Zebrafish
Zebrafish - embryology
Zebrafish Proteins - metabolism
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Summary:A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell–resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at singlecell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2025793118