Overexpression of Long Noncoding RNA H19 Downregulates miR-140-5p and Activates PI3K/AKT Signaling Pathway to Promote Invasion, Migration and Epithelial-Mesenchymal Transition of Ovarian Cancer Cells

Objective. The abnormal expression of LncRNA H19 and miR-140-5p has been linked to ovarian cancer (OC). Whether H19 directly regulates miR-140-5p in ovarian cancer cells has been unclear. In this study, we deeply explored the relationship between H19 and miR-140-5p in ovarian cancer and the mechanis...

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Bibliographic Details
Published in:BioMed research international 2021-06, Vol.2021, p.6619730-13
Main Authors: Xu, Hao, Ding, Yuan, Yang, Xiangying
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Animals
Apoptosis
Base Sequence
Biomedical research
Cancer
Carcinogenesis - genetics
Carcinogenesis - pathology
Cell activation
Cell differentiation
Cell Line, Tumor
Cell Movement - genetics
Cell proliferation
Cellular signal transduction
Development and progression
Down-Regulation - genetics
Epithelial cells
Epithelial-Mesenchymal Transition - genetics
Epithelium
Experiments
Female
Flow cytometry
Gene Expression Regulation, Neoplastic
Genetic aspects
Health aspects
Humans
Medical prognosis
Mesenchyme
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Neoplasm Invasiveness
Oncology, Experimental
Ovarian cancer
Ovarian Neoplasms - genetics
Ovarian Neoplasms - pathology
Phosphatidylinositol 3-Kinases - metabolism
Protein kinases
Proteins
Proto-Oncogene Proteins c-akt - metabolism
RNA
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Signal Transduction
Signaling
Stem cells
Transfection
Tumors
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Summary:Objective. The abnormal expression of LncRNA H19 and miR-140-5p has been linked to ovarian cancer (OC). Whether H19 directly regulates miR-140-5p in ovarian cancer cells has been unclear. In this study, we deeply explored the relationship between H19 and miR-140-5p in ovarian cancer and the mechanism of action in regulating OC progression. Methods. A total of 66 patients with OC admitted to the hospital from June 2017 to June 2019 were selected as the research group (RG), and meanwhile, 60 cases of healthy subjects were selected as the control group (CG). In addition, OC cells and normal ovarian epithelial cells were used to detect H19 and miR-140-5p expression levels and to analyze the effect of H19 on OC cells. The activation of the PI3K/AKT pathway and downstream proteins were analyzed by western blot. Results. H19 was highly expressed while miR-140-5p was lowly expressed in OC patients and cell lines (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2021/6619730