Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea

DNA methylation has been proposed to regulate plant stress resistance. However, the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood. Here, we present single-base maps of the DNA methylomes of mulberry ( Morus notabilis ) le...

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Bibliographic Details
Published in:Horticulture research 2021-07, Vol.8 (1), Article 154
Main Authors: Xin, Youchao, Ma, Bi, Zeng, Qiwei, He, Wenmin, Qin, Meiling, He, Ningjia
Format: Article
Language:English
Subjects:
631/337/176/1988
631/449/2661/2666
Agriculture
Biomedical and Life Sciences
Botrytis cinerea
Deoxyribonucleic acid
Disease resistance
DNA
DNA methylation
Ecology
Gene expression
Gene mapping
Gene silencing
Genes
Inoculation
Life Sciences
Morus notabilis
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plant stress
siRNA
Transposons
Viruses
Woody plants
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Summary:DNA methylation has been proposed to regulate plant stress resistance. However, the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood. Here, we present single-base maps of the DNA methylomes of mulberry ( Morus notabilis ) leaves that were subjected to a mock treatment or inoculation with Botrytis cinerea . Compared with the former, the latter showed decreased mCG and mCHG levels and increased mCHH levels. DNA methylation inhibitors reduced resistance gene methylation levels and enhanced mulberry resistance, suggesting that the hypomethylation of resistance genes affects mulberry resistance to B. cinerea . Virus-induced gene silencing of MnMET1 enhanced the expression of mulberry-resistance genes, thereby increasing the plant’s resistance to B. cinerea . We also found that MITE s play a dominant role in controlling DNA methylation levels. MITE s appear to be the main sources of 24-nt siRNAs that regulate gene expression through the RNA-directed DNA methylation pathway.
ISSN:2662-6810
2052-7276
DOI:10.1038/s41438-021-00588-x