VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine

Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accessi...

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Bibliographic Details
Published in:Horticulture research 2021-07, Vol.8 (1), Article 151
Main Authors: Jiang, Changyue, Wang, Dan, Zhang, Jie, Xu, Yan, Zhang, Chaohong, Zhang, Jianxia, Wang, Xiping, Wang, Yuejin
Format: Article
Language:English
Subjects:
631/337/572/2102
631/449/2169/2674
631/449/2661/2666
631/449/2675
Accumulation
Agriculture
Apoptosis
Biomedical and Life Sciences
Biosynthesis
Cell death
Disease resistance
Ecology
Genes
Grapevines
Homology
Inoculation
Life Sciences
Pathogens
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Pseudomonas syringae
Reactive oxygen species
Regulatory mechanisms (biology)
Resveratrol
Stilbene
Stilbene synthase
Transcription factors
Uncinula necator
Vitis quinquangularis
Yeasts
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Summary:Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator , Pseudomonas syringae , and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9 , VqSTS32 , and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae . These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine.
ISSN:2662-6810
2052-7276
DOI:10.1038/s41438-021-00585-0