Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection

We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation‐induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2021-05, Vol.22 (9), p.1563-1567
Main Authors: Zimmermann, Alexander, Jaber, Qais Z., Koch, Johannes, Riebe, Steffen, Vallet, Cecilia, Loza, Kateryna, Hayduk, Matthias, Steinbuch, Kfir B., Knauer, Shirley K., Fridman, Micha, Voskuhl, Jens
Format: Article
Language:English
Subjects:
aggregation-induced emission
Aminoglycosides
Binding
bioimaging
cationic amphiphiles
Cations
Communication
Communications
Deoxyribonucleic acid
DNA
DNA probes
Electrostatic properties
Emission
Emissions
Fluorescence
Mammalian cells
Morphology
Plasmids
Reagents
Red fluorescent protein
self-assembly
Transfection
transfection agents
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Summary:We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation‐induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ‐potential and dynamic light‐scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold‐standard transfection reagent Lipofectamine® 2000. Transfection tracker: Conjugates of luminophores with aggregation‐induced emission properties linked to the aminoglycoside tobramycin are reported. These compounds reveal an emission “on” behavior upon plasmid‐DNA binding and are able to transfect different mammalian cell lines. The cellular uptake was investigated using the emission properties of the formed plasmid DNA‐vector assemblies.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202000725