Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimpla...

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Published in:International journal of molecular sciences 2021-07, Vol.22 (13), p.6720
Main Authors: Pierzchała, Dorota, Liput, Kamila, Korwin-Kossakowska, Agnieszka, Ogłuszka, Magdalena, Poławska, Ewa, Nawrocka, Agata, Urbański, Paweł, Ciepłoch, Aleksandra, Juszczuk-Kubiak, Edyta, Lepczyński, Adam, Ślaska, Brygida, Kowal, Krzysztof, te Pas, Marinus F. W., Śmiech, Magdalena, Leszczyński, Paweł, Taniguchi, Hiroaki, Fraser, Leyland, Sobiech, Przemysław, Sachajko, Mateusz, Herudzinska, Magdalena, Pareek, Chandra S., Pierzchała, Mariusz
Format: Article
Language:English
Subjects:
Embryos
Endometrium
Gels
Hogs
Identification
Implantation
Mass spectrometry
Mass spectroscopy
Metabolic pathways
Metabolism
Pregnancy
Proteins
Proteomes
Recognition
Scientific imaging
Software
Swine
Uterus
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Summary:The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGOTM approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22136720