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Field Performance of a Rapid Diagnostic Test for the Serodiagnosis of Abdominal Cystic Echinococcosis in the Peruvian Highlands
We evaluated the performance of a commercial rapid diagnostic test (RDT) in a field setting for the diagnosis of abdominal cystic echinococcosis (CE) using sera collected during an ultrasound population screening in a highly endemic region of the Peruvian Andes. Abdominal CE was investigated by ultr...
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Published in: | The American journal of tropical medicine and hygiene 2021-07, Vol.105 (1), p.181-187 |
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creator | Manciulli, Tommaso Enríquez-Laurente, Raul Tamarozzi, Francesca Lissandrin, Raffaella Elizalde, Maira Sedano, Cesar Bardales, Karina Vola, Ambra De Silvestri, Annalisa Tinelli, Carmine Brunetti, Enrico Santivanez, Saul Mariconti, Mara |
description | We evaluated the performance of a commercial rapid diagnostic test (RDT) in a field setting for the diagnosis of abdominal cystic echinococcosis (CE) using sera collected during an ultrasound population screening in a highly endemic region of the Peruvian Andes. Abdominal CE was investigated by ultrasonography. Sera collected from individuals with abdominal CE (cases) and age- and gender-matched volunteers with no abdominal CE (controls) were tested independently in two laboratories (Peru and Italy) using the VIRapid® HYDATIDOSIS RDT and RIDASCREEN® Echinococcus IgG enzyme-linked immunosorbent assay. Performance indexes of single and serially combined tests were calculated and applied to hypothetical screening and clinical scenarios. Test concordance was also evaluated. Prevalence of abdominal CE was 6.00% (33 of 546) by ultrasound. Serum was obtained from 33 cases and 81 controls. The VIRapid test showed similar sensitivity (76% versus 74%) and lower specificity (79% versus 96%) than results obtained in a hospital setting. RDTs showed better performance when excluding subjects reporting surgery for CE and if weak bands were considered negative. Concordance between tests was moderate to very good. In hypothetical screening scenarios, ultrasound alone or confirmed by RDTs provided more reliable prevalence figures than serology alone, which overestimated it by 5 to 20 times. In a simulation of case diagnosis with pre-test probability of CE of 50%, positive and negative post-test probabilities of the VIRapid test were 78% and 22%, respectively. The application of the VIRapid test alone would not be reliable for the assessment of population prevalence of CE, but could help clinical decision making in resource-limited settings. |
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Abdominal CE was investigated by ultrasonography. Sera collected from individuals with abdominal CE (cases) and age- and gender-matched volunteers with no abdominal CE (controls) were tested independently in two laboratories (Peru and Italy) using the VIRapid® HYDATIDOSIS RDT and RIDASCREEN® Echinococcus IgG enzyme-linked immunosorbent assay. Performance indexes of single and serially combined tests were calculated and applied to hypothetical screening and clinical scenarios. Test concordance was also evaluated. Prevalence of abdominal CE was 6.00% (33 of 546) by ultrasound. Serum was obtained from 33 cases and 81 controls. The VIRapid test showed similar sensitivity (76% versus 74%) and lower specificity (79% versus 96%) than results obtained in a hospital setting. RDTs showed better performance when excluding subjects reporting surgery for CE and if weak bands were considered negative. Concordance between tests was moderate to very good. In hypothetical screening scenarios, ultrasound alone or confirmed by RDTs provided more reliable prevalence figures than serology alone, which overestimated it by 5 to 20 times. In a simulation of case diagnosis with pre-test probability of CE of 50%, positive and negative post-test probabilities of the VIRapid test were 78% and 22%, respectively. The application of the VIRapid test alone would not be reliable for the assessment of population prevalence of CE, but could help clinical decision making in resource-limited settings.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.21-0045</identifier><identifier>PMID: 34029208</identifier><language>eng</language><publisher>United States: Institute of Tropical Medicine</publisher><subject>Abdomen ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antibodies, Helminth - blood ; Child ; Child, Preschool ; Diagnostic tests ; Diagnostic Tests, Routine - methods ; Echinococcosis - diagnosis ; Echinococcosis - epidemiology ; Echinococcosis - therapy ; Echinococcus - genetics ; Epidemiological Monitoring ; Female ; Humans ; Male ; Middle Aged ; Peru - epidemiology ; Serologic Tests - methods ; Ultrasonic imaging ; Young Adult</subject><ispartof>The American journal of tropical medicine and hygiene, 2021-07, Vol.105 (1), p.181-187</ispartof><rights>Copyright Institute of Tropical Medicine Jul 2021</rights><rights>The American Society of Tropical Medicine and Hygiene 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-20f6c84b832ad33cedd0b72b38cb0044f40785cd6cd925484e3b1e3d204c3b5e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274789/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274789/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34029208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manciulli, Tommaso</creatorcontrib><creatorcontrib>Enríquez-Laurente, Raul</creatorcontrib><creatorcontrib>Tamarozzi, Francesca</creatorcontrib><creatorcontrib>Lissandrin, Raffaella</creatorcontrib><creatorcontrib>Elizalde, Maira</creatorcontrib><creatorcontrib>Sedano, Cesar</creatorcontrib><creatorcontrib>Bardales, Karina</creatorcontrib><creatorcontrib>Vola, Ambra</creatorcontrib><creatorcontrib>De Silvestri, Annalisa</creatorcontrib><creatorcontrib>Tinelli, Carmine</creatorcontrib><creatorcontrib>Brunetti, Enrico</creatorcontrib><creatorcontrib>Santivanez, Saul</creatorcontrib><creatorcontrib>Mariconti, Mara</creatorcontrib><title>Field Performance of a Rapid Diagnostic Test for the Serodiagnosis of Abdominal Cystic Echinococcosis in the Peruvian Highlands</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>We evaluated the performance of a commercial rapid diagnostic test (RDT) in a field setting for the diagnosis of abdominal cystic echinococcosis (CE) using sera collected during an ultrasound population screening in a highly endemic region of the Peruvian Andes. Abdominal CE was investigated by ultrasonography. Sera collected from individuals with abdominal CE (cases) and age- and gender-matched volunteers with no abdominal CE (controls) were tested independently in two laboratories (Peru and Italy) using the VIRapid® HYDATIDOSIS RDT and RIDASCREEN® Echinococcus IgG enzyme-linked immunosorbent assay. Performance indexes of single and serially combined tests were calculated and applied to hypothetical screening and clinical scenarios. Test concordance was also evaluated. Prevalence of abdominal CE was 6.00% (33 of 546) by ultrasound. Serum was obtained from 33 cases and 81 controls. The VIRapid test showed similar sensitivity (76% versus 74%) and lower specificity (79% versus 96%) than results obtained in a hospital setting. RDTs showed better performance when excluding subjects reporting surgery for CE and if weak bands were considered negative. Concordance between tests was moderate to very good. In hypothetical screening scenarios, ultrasound alone or confirmed by RDTs provided more reliable prevalence figures than serology alone, which overestimated it by 5 to 20 times. In a simulation of case diagnosis with pre-test probability of CE of 50%, positive and negative post-test probabilities of the VIRapid test were 78% and 22%, respectively. The application of the VIRapid test alone would not be reliable for the assessment of population prevalence of CE, but could help clinical decision making in resource-limited settings.</description><subject>Abdomen</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diagnostic tests</subject><subject>Diagnostic Tests, Routine - methods</subject><subject>Echinococcosis - diagnosis</subject><subject>Echinococcosis - epidemiology</subject><subject>Echinococcosis - therapy</subject><subject>Echinococcus - genetics</subject><subject>Epidemiological Monitoring</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Peru - epidemiology</subject><subject>Serologic Tests - methods</subject><subject>Ultrasonic imaging</subject><subject>Young Adult</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc1P3DAQxS3Uqiy0R66VJS69BPwVx7lUQlu-JKQiSs-WYzsbrxJ7sRMkTvzrOLsUQQ_WHOY3z_PmAXCE0QkjvD5V63HoTgguEGLlHlhgVvECc1Z-AguEEClqTqt9cJDSGiEsCMZfwD5liNQEiQV4vnC2N_DWxjbEQXltYWihgndq4wz85dTKhzQ6De9tGmFm4NhZ-MfGYHY9l-aBs8aEwXnVw-XTFj_XnfNBB623iPPbufzN9OiUh1du1fXKm_QVfG5Vn-y313oI_l6c3y-vipvfl9fLs5tCM1yOBUEt14I1ghJlKNXWGNRUpKFCN9k4axmqRKkN16YmJRPM0gZbaghimjalpYfg5053MzWDNdr6MapebqIbVHySQTn5seNdJ1fhUQpSsUrUWeDHq0AMD1M-hhxc0rbPLmyYkiQlJflhOqPH_6HrMMV8nJniKK_KEc9UsaN0DClF274tg5Gco5XbaCXBco4289_fO3ij_2VJXwDi_KHi</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Manciulli, Tommaso</creator><creator>Enríquez-Laurente, Raul</creator><creator>Tamarozzi, Francesca</creator><creator>Lissandrin, Raffaella</creator><creator>Elizalde, Maira</creator><creator>Sedano, Cesar</creator><creator>Bardales, Karina</creator><creator>Vola, Ambra</creator><creator>De Silvestri, Annalisa</creator><creator>Tinelli, Carmine</creator><creator>Brunetti, Enrico</creator><creator>Santivanez, Saul</creator><creator>Mariconti, Mara</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210701</creationdate><title>Field Performance of a Rapid Diagnostic Test for the Serodiagnosis of Abdominal Cystic Echinococcosis in the Peruvian Highlands</title><author>Manciulli, Tommaso ; 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Abdominal CE was investigated by ultrasonography. Sera collected from individuals with abdominal CE (cases) and age- and gender-matched volunteers with no abdominal CE (controls) were tested independently in two laboratories (Peru and Italy) using the VIRapid® HYDATIDOSIS RDT and RIDASCREEN® Echinococcus IgG enzyme-linked immunosorbent assay. Performance indexes of single and serially combined tests were calculated and applied to hypothetical screening and clinical scenarios. Test concordance was also evaluated. Prevalence of abdominal CE was 6.00% (33 of 546) by ultrasound. Serum was obtained from 33 cases and 81 controls. The VIRapid test showed similar sensitivity (76% versus 74%) and lower specificity (79% versus 96%) than results obtained in a hospital setting. RDTs showed better performance when excluding subjects reporting surgery for CE and if weak bands were considered negative. Concordance between tests was moderate to very good. In hypothetical screening scenarios, ultrasound alone or confirmed by RDTs provided more reliable prevalence figures than serology alone, which overestimated it by 5 to 20 times. In a simulation of case diagnosis with pre-test probability of CE of 50%, positive and negative post-test probabilities of the VIRapid test were 78% and 22%, respectively. The application of the VIRapid test alone would not be reliable for the assessment of population prevalence of CE, but could help clinical decision making in resource-limited settings.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>34029208</pmid><doi>10.4269/ajtmh.21-0045</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Abdomen Adolescent Adult Aged Aged, 80 and over Animals Antibodies, Helminth - blood Child Child, Preschool Diagnostic tests Diagnostic Tests, Routine - methods Echinococcosis - diagnosis Echinococcosis - epidemiology Echinococcosis - therapy Echinococcus - genetics Epidemiological Monitoring Female Humans Male Middle Aged Peru - epidemiology Serologic Tests - methods Ultrasonic imaging Young Adult |
title | Field Performance of a Rapid Diagnostic Test for the Serodiagnosis of Abdominal Cystic Echinococcosis in the Peruvian Highlands |
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