Screening and Purification of Natural Products from Actinomycetes that Induce a “Rounded” Morphological Phenotype in Fission Yeast

This study was designed to identify and investigate bioactive natural product compounds that alter the cellular shape of the fission yeast Schizosaccharomyces pombe and induce a “rounded” or “small” cellular morphological phenotype. Bioassays using a range of antifungal agents against a multidrug-se...

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Bibliographic Details
Published in:Natural products and bioprospecting 2021-08, Vol.11 (4), p.431-445
Main Authors: Lewis, Richard Alexander, Devi, Jenileima, Green, Katherine, Li, Juanjuan, Hopkins, Adam, Hayles, Jacqueline, Nurse, Paul, Errington, Jeff, Allenby, Nicholas Edward Ellis
Format: Article
Language:English
Subjects:
Actinomycetes
Antifungal agents
Biomedical and Life Sciences
Bioorganic Chemistry
Candicidin
Fission
Food Science
Fungicides
Genotype & phenotype
Life Sciences
Medicinal Chemistry
Morphology
Natural products
Original
Original Article
Pharmacology/Toxicology
Phenotypes
Plant Biochemistry
Plant Sciences
Polyenes
Streptothricin
Taxonomy
Yeast
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Summary:This study was designed to identify and investigate bioactive natural product compounds that alter the cellular shape of the fission yeast Schizosaccharomyces pombe and induce a “rounded” or “small” cellular morphological phenotype. Bioassays using a range of antifungal agents against a multidrug-sensitive fission yeast strain, SAK950 showed that many induced a “rounded” phenotype. We then investigated whether 46 of the actinomycete strains identified in our previous study as inducing a similar phenotype produced antifungal agents of similar classes. We show that five of the strains produced streptothricin and that 26 strains produced polyenes, including fungichromin, filipin and candicidin, the last of which was produced by 24 strains. A taxonomic study of the strains indicated that the majority of the candicidin only producers were Streptomyces hydrogenans and S. albidoflavus whilst those that additionally produced streptothricin were related to S. enissocaesilis . A follow-up study to investigate the natural products made by related strains indicated that they followed a similar pattern. The identification of several compounds from the actinomycete strains similar to the antifungal agents initially tested confirm the validity of an approach using the S. pombe morphological phenotype and actinomycete taxonomy as a predictive tool for natural product identification. Graphic Abstract
ISSN:2192-2195
2192-2209
DOI:10.1007/s13659-021-00304-1