Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress

• Published in: Cell stress & chaperones 2021-07, Vol.26 (4), p.705-720
• Main Authors: Park, Da Som, Gu, Bon-Hee, Park, Yei Ju, Joo, Sang Seok, Lee, Sang-Suk, Kim, Seon-Ho, Kim, Eun Tae, Kim, Dong Hyeon, Lee, Sung Sill, Lee, Shin Ja, Kim, Byeong-Woo, Kim, Myunghoo
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blood; Blood - immunology; Blood - metabolism; Blood Cells - cytology; Blood Cells - immunology; Cancer Research; Cattle; Cell Biology; Domestic animals; Environmental conditions; Gene expression; Heat shock proteins; Heat stress; Heat Stress Disorders - immunology; Heat tolerance; Heat-Shock Response - immunology; Hot Temperature; Hsp70 protein; Hsp90 protein; Immune system; Immunology; Interleukin 10; Interleukin 6; Lactation - immunology; Lactation - physiology; Leukocytes (polymorphonuclear); Livestock; Lymphocytes; Lymphocytes T; Neurosciences; Neutrophils - cytology; Neutrophils - immunology; ORIGINAL PAPER; Peroxidase; Physiological effects; Populations
• ISSN: 1355-8145; 1466-1268
• DOI: 10.1007/s12192-021-01216-2

Heat stress has detrimental effects on livestock via diverse immune and physiological changes; heat-stressed animals are rendered susceptible to diverse diseases. However, there is relatively little information available regarding the altered immune responses of domestic animals in heat stress environments, particularly in cattle steers. This study aimed to determine the changes in the immune responses of Holstein and Jersey steers under heat stress. We assessed blood immune cells and their functions in the steers of two breeds under normal and heat stress conditions and found that immune cell proportions and functions were altered in response to different environmental conditions. Heat stress notably reduced the proportions of CD21⁺MHCII⁺ B cell populations in both breeds. We also observed breed-specific differences. Under heat stress, in Holstein steers, the expression of myeloperoxidase was reduced in the polymorphonuclear cells, whereas heat stress reduced the WC1⁺ γδ T cell populations in Jersey steers. Breed-specific changes were also detected based on gene expression. In response to heat stress, the expression of IL-10 and IL-17A increased in Holstein steers alone, whereas that of IL-6 increased in Jersey steers. Moreover, the mRNA expression pattern of heat shock protein genes such as Hsp70 and Hsp90 was significantly increased in only Holstein steers. Collectively, these results indicate that altered blood immunological profiles may provide a potential explanation for the enhanced susceptibility of heat-stressed steers to disease. The findings of this study provide important information that will contribute to developing new strategies to alleviate the detrimental effects of heat stress on steers.