Comparison of acute inhalation toxicity of sulfuric acid by the inhalation and intratracheal instillation methods

• Published in: Journal of Toxicologic Pathology 2021, Vol.34(3), pp.269-273
• Main Authors: Takeuchi, Kazuya, Kuroda, Yusuke, Numano, Takamasa, Kimura, Masayuki, Hayashi, Seigo, Furukawa, Satoshi
• Format: Article
• Language: English
• Subjects: Abnormalities; acute inhalation toxicity; Acute toxicity; Alveoli; Animals; Body weight; Epithelium; Inhalation; Interferon; intratracheal instillation; Lethal dose; Lungs; Mucosa; Necrosis; Respiration; Respiratory system; Rodents; Sensitivity analysis; Short Communication; Sulfuric acid; Toxicity; Trachea
• ISSN: 0914-9198; 1881-915X; 1347-7404
• DOI: 10.1293/tox.2020-0086

Recently, intratracheal instillation has been focused on as a simple, low-cost alternative to the inhalation method. In this study, intratracheal instillation of sulfuric acid, a typical acidic compound, was performed to compare the acute toxicity of acidic compounds that could cause damage to the respiratory system between intratracheal instillation and inhalation. Sulfuric acid was administered to male rats at doses of 0.7, 2, 7, 20, and 60 mg/kg by dividing the total dose into four doses. General condition and body weight were examined up to 14 days after administration, and macropathological and histopathological examinations were performed. The half-lethal dose was then estimated. All animals administered 20 and 60 mg/kg sulfuric acid and one animal administered 2 mg/kg sulfuric acid died within 4 h after administration. No abnormalities were observed in other animals. At 20 and 60 mg/kg, multiple red foci or diffuse red areas were macroscopically observed in the lungs. In these lesions, histopathologically, clefts between the mucosal epithelium and basement membrane and necrosis of the alveolar epithelium were observed. Deaths in these groups may have resulted from lung injury. No notable changes were observed in other animals. Therefore, the half-lethal dose of sulfuric acid by intratracheal instillation was estimated as 7–20 mg/kg. The acute toxicity by intratracheal instillation was evaluated with two-fold sensitivity since the exposure at the half-lethal sulfuric acid concentration in inhalation studies was calculated as 43.2 mg/kg.