The effects of compound centella formula on OxInflammation and silent information regulator 1 in a high‑fat diet/streptozotocin‑induced diabetic kidney disease rat model

The Chinese decoction compound centella formula (CCF) is clinically effective against diabetic kidney disease (DKD), but the exact mechanism remains unclear. The present study aimed to investigate the effects of CCF on OxInflammation and silent information regulator 1 (SIRT1) levels in rats with str...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2021-09, Vol.22 (3), Article 962
Main Authors: Zhu, Qin, Li, Xiao-Hong, Chen, Hong-Yu, Jin, Qin-Yang
Format: Article
Language:English
Subjects:
Biological products
Content analysis
Diabetes
Diabetic nephropathy
Diet
Drug dosages
Ethanol
Experiments
Feeds
Inflammation
Kidney diseases
Laboratory animals
Oxidative stress
Pathogenesis
Pathogens
Solvents
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Summary:The Chinese decoction compound centella formula (CCF) is clinically effective against diabetic kidney disease (DKD), but the exact mechanism remains unclear. The present study aimed to investigate the effects of CCF on OxInflammation and silent information regulator 1 (SIRT1) levels in rats with streptozotocin (STZ)-induced diabetes. Sprague-Dawley rats were divided into CCF, losartan, diabetic control (DC) and normal control (NC) groups (n=7). Except for the NC, all subgroups of rats were fed a high-fat diet for 112 days and received a single intraperitoneal injection of 35 mg/kg STZ on day 29. All rats were sacrificed on day 112. High-performance liquid chromatography was performed to analyse asiaticoside, astragaloside and triptolide levels in CCF (0.3400, 0.0640 and 0.0001 mg/ml, respectively). Fasting blood glucose, urine protein-to-creatinine ratio, serum creatinine and blood urea nitrogen were quantified. Periodic acid Schiff staining, H&E staining and transmission electron microscopy were used to examine kidney pathological changes. The mRNA and protein expression levels of SIRT1 in renal tissues were analysed by reverse transcription-quantitative PCR, western blotting and immunohistochemistry. Oxidative stress was evaluated by measuring the levels of superoxide dismutase (SOD), malondialdehyde (MDA) and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) in renal tissues. TNF-α and NF-κB p65 subunit in renal tissues were assessed for inflammation. Compared with the rats in the NC group, the rats in the DC group exhibited renal injury with proteinuria, decreased expression levels of SIRT1 and SOD (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2021.10394