The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma

Normal stromal cells surrounding the tumor parenchyma, such as the extracellular matrix (ECM), normal fibroblasts, mesenchymal stromal cells, and osteoblasts, play a significant role in the progression of cancers. However, the role of gingival and periodontal ligament tissue-derived stromal cells in...

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Bibliographic Details
Published in:Cancers 2021-07, Vol.13 (14), p.3491
Main Authors: Omori, Haruka, Shan, Qiusheng, Takabatake, Kiyofumi, Nakano, Keisuke, Kawai, Hotaka, Sukegawa, Shintaro, Tsujigiwa, Hidetsugu, Nagatsuka, Hitoshi
Format: Article
Language:English
Subjects:
Acid phosphatase (tartrate-resistant)
Antibiotics
Bioinformatics
Cancer
Cell migration
Cell proliferation
Connective tissue
Experiments
Extracellular matrix
Fibroblasts
Gingiva
Immunofluorescence
Immunohistochemistry
Ligaments
Mesenchyme
Oral squamous cell carcinoma
Parenchyma
Periodontal ligament
Squamous cell carcinoma
Stromal cells
Surgery
Tongue
Tumors
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Summary:Normal stromal cells surrounding the tumor parenchyma, such as the extracellular matrix (ECM), normal fibroblasts, mesenchymal stromal cells, and osteoblasts, play a significant role in the progression of cancers. However, the role of gingival and periodontal ligament tissue-derived stromal cells in OSCC progression is unclear. In this study, the effect of G-SCs and P-SCs on the differentiation, proliferation, invasion, and migration of OSCC cells in vitro was examined by Giemsa staining, Immunofluorescence (IF), (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS), invasion, and migration assays. Furthermore, the effect of G-SCs and P-SCs on the differentiation, proliferation, and bone invasion by OSCC cells in vivo was examined by hematoxylin-eosin (HE) staining, immunohistochemistry (IHC), and tartrate-resistant acid phosphatase (TRAP) staining, respectively. Finally, microarray data and bioinformatics analyses identified potential genes that caused the different effects of G-SCs and P-SCs on OSCC progression. The results showed that both G-SCs and P-SCs inhibited the differentiation and promoted the proliferation, invasion, and migration of OSCC in vitro and in vivo. In addition, genes, including CDK1, BUB1B, TOP2A, DLGAP5, BUB1, and CCNB2, are probably involved in causing the different effects of G-SCs and P-SCs on OSCC progression. Therefore, as a potential regulatory mechanism, both G-SCs and P-SCs can promote OSCC progression.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13143491