Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations

Cocaine- and amphetamine-regulated transcript (CART) is a peptide suggested to play a role in gastrointestinal tract tissue reaction to pathology. Gastric ulceration is a common disorder affecting huge number of people, and additionally, it contributes to the loss of pig livestock production. Import...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-07, Vol.22 (14), p.7437
Main Authors: Zalecki, Michal, Plywacz, Adrianna, Antushevich, Hanna, Franke-Radowiecka, Amelia
Format: Article
Language:English
Subjects:
Amphetamines
Animals
Cocaine
Cocaine- and amphetamine-regulated transcript protein
Confocal microscopy
Digestive system
Enteric nervous system
Experiments
Gastrointestinal system
Gastrointestinal tract
Gene expression
Hogs
Immunofluorescence
Livestock
Motility
Muscles
Nervous system
Pathology
Software
Stomach
Swine
Transcription
Ulcers
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Summary:Cocaine- and amphetamine-regulated transcript (CART) is a peptide suggested to play a role in gastrointestinal tract tissue reaction to pathology. Gastric ulceration is a common disorder affecting huge number of people, and additionally, it contributes to the loss of pig livestock production. Importantly, ulceration as a focal disruption affecting deeper layers of the stomach wall differs from other gastrointestinal pathologies and should be studied individually. The pig’s gastrointestinal tract, due to its many similarities to the human counterpart, provides a valuable experimental model for studying digestive system pathologies. To date, the role of CART in gastric ulceration and the expression of the gene encoding CART in porcine gastrointestinal tube are completely unknown. Therefore, we aimed to verify the changes in the CART expression by Q-PCR (gene encoding CART in the tissue) and double immunofluorescence staining combined with confocal microscopy (CART immunofluorescence in enteric nervous system) in the porcine stomach tissues adjacent to gastric ulcerations. Surprisingly, we found that gastric ulcer caused a significant decrease in the expression of CART-encoding gene and huge reduction in the percentage of CART-immunofluorescent myenteric perikarya and neuronal fibers located within the circular muscle layer. Our results indicate a unique CART-dependent gastric response to ulcer disease.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22147437