Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer's Disease Hallmarks in SAMP8 Mice

• Published in: Nutrients 2021-07, Vol.13 (7), p.2369
• Main Authors: Rosell-Cardona, Cristina, Griñan-Ferré, Christian, Pérez-Bosque, Anna, Polo, Javier, Pallàs, Mercè, Amat, Concepció, Moretó, Miquel, Miró, Lluïsa
• Format: Article
• Language: English
• Subjects: Aging; Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Alzheimer Disease - prevention & control; Alzheimer's disease; Amyloid beta-Peptides - metabolism; Amyloid beta-Protein Precursor - metabolism; Amyloid precursor protein; Animal cognition; Animal Feed; Animals; Brain - drug effects; Brain - metabolism; Brain - pathology; Cyclin-dependent kinase 5; Cyclin-Dependent Kinase 5 - metabolism; Cyclin-dependent kinases; Cytokines - genetics; Cytokines - metabolism; Dietary Supplements; Disease Models, Animal; Ethylenediaminetetraacetic acid; Gene expression; Gene Expression Regulation; Glycogen Synthase Kinase 3 beta - metabolism; Inflammation; Inflammation Mediators - metabolism; Interleukin 17; Interleukin 18; Kinases; Laboratory animals; Manufacturers; Markers; Membranes; Microglia - drug effects; Microglia - metabolism; Microglia - pathology; Neurofibrillary Tangles - drug effects; Neurofibrillary Tangles - metabolism; Neurofibrillary Tangles - pathology; Neuropathology; Neurophysiology; Pathogenesis; Peptide Fragments - metabolism; Peptides; Phosphorylation; Plasma; Proteins; Senescence; Signal Transduction; Software; Spray Drying; Sus scrofa; Tau protein; tau Proteins - metabolism; Temperature; Transforming growth factors; β-Site APP-cleaving enzyme 1
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13072369

Alzheimer's disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary supplementation with SDP prevents the neuropathological features of AD. The experiments were performed in 2- and 6-month-old SAMP8 mice fed a control diet, or a diet supplemented with 8% SDP, for 4 months. AD brain molecular markers were determined by Western blot and real-time PCR. Senescent mice showed reduced levels of p-GSK3β (Ser9) and an increase in p-CDK5, p-tau (Ser396), sAPPβ, and the concentration of Aβ (all < 0.05). SDP prevented these effects of aging and reduced levels (all < 0.05). Senescence increased the expression of and and pro-inflammatory cytokines ( and ; all < 0.05); these changes were prevented by SDP supplementation. Moreover, SDP increased expression ( < 0.05). Furthermore, in aged mice, the gene expression levels of the microglial activation markers , , and were increased, and SDP prevented these increases (all < 0.05). Thus, dietary SDP might delay AD onset by reducing its hallmarks in senescent mice.