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An investigation of alkaline phosphatase enzymatic activity after electrospinning and electrospraying

The high target specificity and multifunctionality of proteins has led to great interest in their clinical use. To this end, the development of delivery systems capable of preserving their bioactivity and improving bioavailability is pivotal to achieve high effectiveness and satisfactory therapeutic...

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Bibliographic Details
Published in:Journal of drug delivery science and technology 2021-08, Vol.64, p.None-None, Article 102592
Main Authors: Onyekuru, Lesley C., Moreira, Anabela, Zhang, Jiazhe, Angkawinitwong, Ukrit, Costa, Pedro F., Brocchini, Steve, Williams, Gareth R.
Format: Article
Language:English
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Summary:The high target specificity and multifunctionality of proteins has led to great interest in their clinical use. To this end, the development of delivery systems capable of preserving their bioactivity and improving bioavailability is pivotal to achieve high effectiveness and satisfactory therapeutic outcomes. Electrohydrodynamic (EHD) techniques, namely electrospinning and electrospraying, have been widely explored for protein encapsulation and delivery. In this work, monoaxial and coaxial electrospinning and electrospraying were used to encapsulate alkaline phosphatase (ALP) into poly(ethylene oxide) fibres and particles, respectively, and the effects of the processing techniques on the integrity and bioactivity of the enzyme were assessed. A full morphological and physicochemical characterisation of the blend and core-shell products was performed. ALP was successfully encapsulated within monolithic and core-shell electrospun fibres and electrosprayed particles, with drug loadings and encapsulation efficiencies of up to 21% and 99%, respectively. Monoaxial and coaxial electrospinning were equally effective in preserving ALP function, leading to no activity loss compared to fresh aqueous solutions of the enzyme. While the same result was observed for monoaxial electrospraying, coaxial electrospraying of ALP caused a 40% reduction in its bioactivity, which was attributed to the high voltage (22.5 kV) used during processing. This demonstrates that choosing between blend and coaxial EHD processing for protein encapsulation is not always straightforward, being highly dependent on the chosen therapeutic agent and the effects of the processing conditions on its bioactivity. •Blend and coaxial electrohydrodynamic techniques can be used for alkaline phosphatase (ALP) encapsulation.•Both blend and coaxial electrospinning equally preserve ALP bioactivity at ca. 100%.•ALP activity decreased after coaxial electrospraying, but not blend electrospraying.•The high voltages needed for coaxial electrospraying appear to affect ALP bioactivity.
ISSN:1773-2247
2588-8943
DOI:10.1016/j.jddst.2021.102592