BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma

• Published in: International journal of biological sciences 2021-01, Vol.17 (10), p.2461-2475
• Main Authors: Mao, Chun-Guo, Jiang, Sha-Sha, Wang, Xiao-Yang, Tao, Shao-Lin, Jiang, Bin, Mao, Cheng-Yi, Yang, Yan-Lian, Hu, Zhi-Yuan, Long, Tan, Jin, Hua, Tan, Qun-You, Huang, Yi, Deng, Bo
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Adenocarcinoma of Lung - genetics; Adenocarcinoma of Lung - mortality; Adenocarcinoma of Lung - pathology; Animals; Anoikis; Antibodies; Antiestrogens; B7-H1 Antigen - metabolism; Biomarkers; Blood; Breast cancer; Cancer; Carcinogenesis; Carcinogens; Cell Cycle Proteins - metabolism; Cell growth; Cell Line, Tumor; Cell migration; Cell proliferation; Cell Proliferation - genetics; Crk-Associated Substrate Protein - genetics; Crk-Associated Substrate Protein - metabolism; Disease; Epithelial-Mesenchymal Transition - genetics; Estrogens; Exosomes; Gene Expression Regulation, Neoplastic; Hepatitis; Humans; Invasiveness; Kinases; Lung Neoplasms - genetics; Lung Neoplasms - mortality; Lung Neoplasms - pathology; Lungs; Mass spectrometry; Mass spectroscopy; Mesenchyme; Mice; Mice, Inbred BALB C; Mice, Nude; Nanoparticles; Neoplastic Cells, Circulating - pathology; Nuclei (cytology); Plasmids; Proteins; Proteomics; Rac1 protein; Research Paper; Signal Transduction; Signaling; Software; Surgery; Transcription Factors - metabolism; Tumor cells; Tumors; Xenograft Model Antitumor Assays; Xenografts
• ISSN: 1449-2288; 1449-2288
• DOI: 10.7150/ijbs.61790

We investigated the roles of breast cancer anti-estrogen resistance 1 (BCAR1/p130Cas) in the formation and immunoevasion of invasive circulating tumor cells (CTCs) in lung adenocarcinoma (LUAD). Biomarkers of CTCs including BCAR1 and CD274, were evaluated by the CanPatrol method. Proteomics analysis of LUAD cells and exosomes after BCAR1 overexpression (BCAR1-OE) was performed by mass spectrometry. Cell functions and relevant signaling pathways were investigated after BCAR1 knockdown (BCAR1-KO) or BCAR1-OE in LUAD cells. Lastly, and experiments were performed to confirm the roles of BCAR1 in the formation and immunoevasion of CTCs. High expression of BCAR1 by CTCs correlated with CD274 expression and epithelial-to-mesenchymal transition (EMT). RAC1, together with BCAR1, was found to play an important role in the carcinogenesis of LUAD. RAC1 functioned with BCAR1 to induce EMT and to enhance cell proliferation, colony formation, cell invasion and migration, and anoikis resistance in LUAD cells. BCAR1 up-regulated CD274 expression probably by shuttling the short isoform of BRD4 (BRD4-S) into the nucleus. CTCs, as well as tumor formation, were prohibited in nude mice xenografted with BCAR1-KO cells. The co-expression of BCAR1/RAC1 and BCAR1/CD274 was confirmed in LUAD. BCAR1 expression in LUAD is an indicator of poor prognosis, and it associates with immunoevasion. BCAR1, as a new target for the treatment of LUAD, plays roles in the formation and immunoevasion of invasive CTCs. The mechanism includes triggering EMT via RAC1 signaling and up-regulating CD274 expression by shuttling BRD4-S into the nucleus.