Rhizobia and endophytic bacteria isolated from rainforest fragments within an iron ore mining site of the Eastern Brazilian Amazon

• Published in: Brazilian journal of microbiology 2021-09, Vol.52 (3), p.1461-1474
• Main Authors: Rodríguez-Rodríguez, Raquel Milagros, Guimarães, Amanda Azarias, de Castro, Jordana Luísa, Siqueira, José Oswaldo, Carneiro, Marco Aurélio Carbone, de Souza Moreira, Fatima Maria
• Format: Article
• Language: English
• Subjects: Amplification; Bacteria; Bacteria - classification; Biological activity; Biomedical and Life Sciences; Brazil; Endophytes; Endophytes - classification; Environmental Microbiology - Research Paper; Food Microbiology; Host plants; Iron; Iron compounds; Iron ores; Life Sciences; Macroptilium atropurpureum; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mining; Mycology; Nodulation; Nodules; Paenibacillus; Phylogeny; Rainforest; Rainforests; Reclamation; Rhizobium - classification; Root Nodules, Plant; Soil; Soil investigations; Soil sciences; Soils; Strains (organisms); Symbiosis
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-021-00524-0

The aim of the present study was to isolate and evaluate the diversity of rhizobial and endophytic bacterial strains from undisturbed native rainforests within an iron ore mining site of the Serra Norte de Carajás in the Eastern Brazilian Amazon region to assess their biotechnological utility in reclamation of areas. Experiments were conducted to capture strains from samples of the soil of these forests at the sites Arenito II, Noroeste II, and Sul IV using Macroptilium atropurpureum and Mimosa acutistipula var. ferrea as trap host plants. Only M. atropurpureum nodulated, and the different bacterial strains were isolated from its nodules. There was no difference in the number of nodules among the areas, but the Arenito II bacterial community was the most efficient, indicated by the aboveground biomass production and suitable shoot mass/root mass ratio. Fifty-two (52) bacterial isolates were obtained, distributed in five groups, including nodulating and endophytic bacteria: 32 from Arenito II, 12 from Noroeste II, and 8 from Sul IV. The nodulating Bradyrhizobium genus was common to the three areas, whereas Paraburkholderia was found only in Arenito II. The nodD1 gene was amplified in all the strains of both nodulating genera. Strains of the nodulating genus Methylobacterium were also isolated from the three areas; however, they did not nodulate the host of origin, and their nodD1 gene was not amplified. Endophytic strains were also isolated from the genera Paenibacillus , Pantoea , and Leifsonia in Arenito II, Leifsonia in Noroeste I, and Paenibacillus in Sul IV. The greater nodulation and rhizobial and endophytic bacterial diversity observed in Arenito II were probably due to the more suitable edaphic properties of the area. The isolated strains were incorporated in the collection of the Department of Soil Science of UFLA and will be investigated in relation to their symbiotic characteristics with native host plants, as well as their ability to perform other biological processes.