Identification of the molecular function of tripartite motif containing 58 in human lung cancer

Lung cancer is a major public health problem worldwide, with a high associated incidence and mortality. In the present study, novel epigenetic signatures were identified through genome-wide DNA methylation microarrays. The results revealed that tripartite motif containing 58 (TRIM58), a potential tu...

Full description

Saved in:
Bibliographic Details
Published in:Oncology letters 2021-09, Vol.22 (3), p.1, Article 685
Main Author: Shi, Yuan-Xiang
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Biotechnology
Cancer
Cell growth
China
Comparative analysis
Datasets
Diagnosis
DNA methylation
DNA microarrays
Epigenetic inheritance
Epigenetics
Gene expression
Genes
Genomes
Genomics
Health aspects
Lung cancer
Medical diagnosis
Medical prognosis
Methylation
Mortality
Oncology
Proteins
Reagents
Scientific equipment and supplies industry
Signal transduction
Tumors
United States
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lung cancer is a major public health problem worldwide, with a high associated incidence and mortality. In the present study, novel epigenetic signatures were identified through genome-wide DNA methylation microarrays. The results revealed that tripartite motif containing 58 (TRIM58), a potential tumor suppressor gene exhibited high methylation and low expression in lung cancer tissue samples compared with normal tissues. Receiver operating characteristic curve analysis demonstrated that TRIM58 may be a promising early diagnostic indicator of lung cancer. In addition, the present study analyzed the role of TRIM58 in tumorigenesis and development in lung cancer A549 cells. Wound healing assay and transwell migration assay were used to investigate cell migration, and flow cytometry analysis was used to detect apoptosis. Silencing TRIM58 accelerated the proliferation and migration of lung cancer cells. In contrast, the overexpression of TRIM58 significantly inhibited the proliferation and migration of lung cancer cells and promoted apoptosis. Gene set enrichment analysis revealed that TRIM58 expression was negatively correlated with MYC targets, [G.sub.2]M checkpoints and the mTORC1 signaling pathway. These results of the present study suggested that TRIM58, a potential tumor suppressor gene may serve as a novel diagnostic biomarker and therapeutic target in human lung cancer. Key words: lung cancer, early diagnosis, malignant phenotype, tripartite motif containing 58
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2021.12946