Protective Effect of Luteolin on D-Galactosamine (D-Gal)/Lipopolysaccharide (LPS) Induced Hepatic Injury by in Mice

• Published in: BioMed research international 2021, Vol.2021 (1), p.2252705-2252705
• Main Authors: Pu, Yiwei, Yang, Zhaocong, Mo, Xuming
• Format: Article
• Language: English
• Subjects: Alanine; Alanine transaminase; Alanine Transaminase - blood; Animals; Antibodies; Aspartate Aminotransferases - blood; Cytokines; Cytokines - blood; D-Galactosamine; Distilled water; Enzymes; Galactosamine; Histopathology; IL-1β; Inflammation; Injuries; Interleukin 6; Lipopolysaccharides; Liver; Liver - drug effects; Liver - enzymology; Liver - injuries; Liver - pathology; Luteolin - pharmacology; Male; Mice; Mice, Inbred C57BL; Morphology; NF-κB protein; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Protective Agents - pharmacology; Proteins; Transaminase; Transcription Factor RelA - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vegetable oils; Western blotting
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2021/2252705

To observe the effects of luteolin on galactosamine (D-Gal)/lipopolysaccharide (LPS) induced liver injury in mice. Male C57BL/6 mice were randomly divided into 4 groups: normal control group, D-GaI/LPS group, D-GaI/LPS + luteolin (Lu, 20 mg/kg), and D-GaI/LPS + luteolin (Lu, 40 mg/kg). Mice in the normal control group and D-GaI/LPS group were given distilled water while other groups were given drugs in 7 days by gavage. 4 hours after the continuous administration, Gal (700 mg/kg) and LPS (10 mg/kg) were injected intraperitoneally. Mice in the normal control group were given the same volume of vegetable oil solution. 24 h after the establishment of the mice model, blood and liver samples were collected. Hematoxylin (HE) staining was used to observe the changes of hepatic histopathology. Alanine aminotransferase (ALT) and glutamic oxalacetic transaminase (AST) in serum, interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor (TNF-α) were measured by related kits. Western blotting was used to demonstrate the expression levels of related inflammation proteins. Lu significantly reduced levels of proinflammatory cytokines including interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) in serum and liver. Lu restored the pathological changes after galactosamine (D-Gal)/lipopolysaccharide (LPS) treatment. In addition, Lu regulated proteins levels of the NLRP3/NF-κB pathway in liver. Lu exhibited therapeutical effects on D-GaI/LPS induced liver injury in mice which might be related to the regulation of the NLRP3/NF-κB pathway.