EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines

Disease-relevant human induced pluripotent stem cells (iPSCs) are generated worldwide for research purposes; however, without robust and practical ethical, legal, and quality standards, there is a high risk that their true potential will not be realized. Best practices for tissue procurement, iPSC r...

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Published in:Stem cell reports 2021-08, Vol.16 (8), p.1853-1867
Main Authors: Steeg, Rachel, Mueller, Sabine C, Mah, Nancy, Holst, Bjørn, Cabrera-Socorro, Alfredo, Stacey, Glyn N, De Sousa, Paul A, Courtney, Aidan, Zimmermann, Heiko
Format: Article
Language:English
Subjects:
Biological Specimen Banks - ethics
Biological Specimen Banks - standards
Biological Specimen Banks - statistics & numerical data
Cell Culture Techniques - methods
Cell Culture Techniques - standards
Cell Differentiation - genetics
Cell Line
Cellular Reprogramming - genetics
Cryopreservation - methods
Europe
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Quality Control
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cited_by cdi_FETCH-LOGICAL-c474t-48724a1d7296cc484a5159bbe5d5ddc722d6ce03b55872f7aa542356606c748a3
cites cdi_FETCH-LOGICAL-c474t-48724a1d7296cc484a5159bbe5d5ddc722d6ce03b55872f7aa542356606c748a3
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container_issue 8
container_start_page 1853
container_title Stem cell reports
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creator Steeg, Rachel
Mueller, Sabine C
Mah, Nancy
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Zimmermann, Heiko
description Disease-relevant human induced pluripotent stem cells (iPSCs) are generated worldwide for research purposes; however, without robust and practical ethical, legal, and quality standards, there is a high risk that their true potential will not be realized. Best practices for tissue procurement, iPSC reprogramming, day-to-day cultivation, quality control, and data management aligned with an ethical and legal framework must be included into daily operations to ensure their promise is maximized. Here we discuss key learning experiences from 7 years of operating the European Bank for induced Pluripotent Stem Cells (EBiSC) and recommend how to incorporate solutions into a daily management framework.
doi_str_mv 10.1016/j.stemcr.2021.07.009
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source ScienceDirect (Online service); PubMed Central
subjects Biological Specimen Banks - ethics
Biological Specimen Banks - standards
Biological Specimen Banks - statistics & numerical data
Cell Culture Techniques - methods
Cell Culture Techniques - standards
Cell Differentiation - genetics
Cell Line
Cellular Reprogramming - genetics
Cryopreservation - methods
Europe
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Quality Control
title EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
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