Dexmedetomidine Directs T Helper Cells toward Th1 Cell Differentiation via the STAT1-T-Bet Pathway

Dexmedetomidine is an α2 adrenergic receptor agonist that has been reported to modulate the polarization of CD4+ T cells. However, the underlying mechanisms by which dexmedetomidine induces T-helper 1 (Th1) cell differentiation remain poorly understood. The aim of this study was to explore the poten...

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Bibliographic Details
Published in:BioMed research international 2021, Vol.2021 (1), p.3725316-3725316
Main Authors: Lei, Daoyun, Liu, Li, Xie, Songhui, Ji, Haiyan, Guo, Yanxing, Ma, Tieliang, Han, Chao
Format: Article
Language:English
Subjects:
Adrenergic receptors
Analysis
Antibodies
CD28 antigen
CD3 antigen
CD4 antigen
Cell differentiation
Cytokines
Dexmedetomidine
Differentiation (biology)
Dosage and administration
Enzyme-linked immunosorbent assay
Flow cytometry
Fludarabine
Gene expression
Health aspects
Helper cells
Immune response
Infections
Interleukin 4
Lymphocytes
Lymphocytes T
Membranes
Methods
Patient outcomes
Phosphorylation
Polymerase chain reaction
Receptors (physiology)
Signal transduction
Stat1 protein
Statistical analysis
T cells
Transcription
Trauma
Western blotting
γ-Interferon
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Summary:Dexmedetomidine is an α2 adrenergic receptor agonist that has been reported to modulate the polarization of CD4+ T cells. However, the underlying mechanisms by which dexmedetomidine induces T-helper 1 (Th1) cell differentiation remain poorly understood. The aim of this study was to explore the potential mechanisms through which dexmedetomidine can induce Th1 cell differentiation. Purified CD4+ T cells were stimulated with anti-CD3/anti-CD28 and then treated with dexmedetomidine. Flow cytometry analysis was adopted to measure the concentration of Th1 cells. Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (qPCR) were performed to detect protein levels and mRNA expression, respectively, of IFN-γ and IL-4. Western blotting was used to determine the phosphorylation of signal transducer and activator of transcription 1 (STAT1) and T-bet expression. The Th1 cell subset and IFN-γ levels were elevated in the dexmedetomidine-induced CD4+ T cells. Dexmedetomidine enhanced the phosphorylation of STAT1 and the expression of T-bet in the CD4+ T cells. Atipamezole (an α2 adrenergic antagonist) and fludarabine (a STAT1 inhibitor) reversed the dexmedetomidine-induced Th1 cell differentiation. These results suggested that dexmedetomidine induced Th1 cell differentiation via the STAT1-T-bet signaling pathway.
ISSN:2314-6133
2314-6141
DOI:10.1155/2021/3725316