An outbreak of ertapenem-resistant, carbapenemase-negative and porin-deficient ESBL-producing Klebsiella pneumoniae complex

Carbapenem-resistant is an emerging healthcare-associated pathogen with dynamic molecular epidemiology. This study presents a retrospective analysis of the distribution and antibiotic resistance patterns of ertapenem-resistant ESBL-producing strains recovered during an outbreak from 2012 to 2014 in...

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Bibliographic Details
Published in:Germs (Bucureşti) 2021-06, Vol.11 (2), p.199-210
Main Authors: Matovina, Mihaela, Abram, Maja, Repac-Antić, Davorka, Knežević, Samira, Bubonja-Šonje, Marina
Format: Article
Language:English
Subjects:
Analysis
Antibacterial agents
Bacterial pneumonia
Beta lactamases
Cloxacillin
Drug resistance in microorganisms
Epidemiology
Original
Pneumonia
Porins
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Summary:Carbapenem-resistant is an emerging healthcare-associated pathogen with dynamic molecular epidemiology. This study presents a retrospective analysis of the distribution and antibiotic resistance patterns of ertapenem-resistant ESBL-producing strains recovered during an outbreak from 2012 to 2014 in a Croatian University hospital. We aimed to estimate genetic relatedness of clinical isolates and underlying mechanisms that conferred the ertapenem-resistant phenotype. Expression analysis of genes involved in the antibiotic resistance showed reduced expression of major non-selective porin channel OmpK35. Reduced expression of OmpK36 porin channel in isolates resistant to at least one more carbapenem, apart from the ertapenem, was found to a lesser degree. Pulsed-field gel electrophoresis analysis of genomic DNA revealed that almost all isolates belonged to the same genetic clone. Caution regarding ertapenem-resistant, carbapenemase-negative porin-deficient mutants of is required as they are widespread, and under selective pressure this could result in a local clonal outbreak.
ISSN:2248-2997
2248-2997
DOI:10.18683/germs.2021.1257