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Perturbations in cardiac metabolism in a human model of acute myocardial ischaemia
Introduction Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to define these metabolic perturbations on a metabolome-wide scale. Objectives This st...
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| Published in: | Metabolomics 2021-09, Vol.17 (9), p.76-76, Article 76 |
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| container_start_page | 76 |
| container_title | Metabolomics |
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| creator | Chacko, Sanoj Mamas, Mamas A. El-Omar, Magdi Simon, David Haseeb, Sohaib Fath-ordoubadi, Farzin Clarke, Bernard Neyses, Ludwig Dunn, Warwick B. |
| description | Introduction
Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to define these metabolic perturbations on a metabolome-wide scale.
Objectives
This study was designed to identify cardiac metabolic changes in serum during the first 5 min following early myocardial ischaemia in humans, applying an untargeted metabolomics approach.
Methods
Peripheral venous samples were collected from 46 patients in a discovery study (DS) and a validation study (VS) (25 for DS, 21 for VS). Coronary sinus venous samples were collected from 7 patients (4 for DS, 3 for VS). Acute myocardial ischaemia was induced by transient coronary occlusion during percutaneous coronary intervention (PCI). Plasma samples were collected at baseline (prior to PCI) and at 1 and 5 min post-coronary occlusion. Samples were analyzed by Ultra Performance Liquid Chromatography-Mass Spectrometry in an untargeted metabolomics approach.
Results
The study observed changes in the circulating levels of metabolites at 1 and 5 min following transient coronary ischaemia. Both DS and VS identified 54 and 55 metabolites as significant (P |
| doi_str_mv | 10.1007/s11306-021-01827-x |
| format | article |
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Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to define these metabolic perturbations on a metabolome-wide scale.
Objectives
This study was designed to identify cardiac metabolic changes in serum during the first 5 min following early myocardial ischaemia in humans, applying an untargeted metabolomics approach.
Methods
Peripheral venous samples were collected from 46 patients in a discovery study (DS) and a validation study (VS) (25 for DS, 21 for VS). Coronary sinus venous samples were collected from 7 patients (4 for DS, 3 for VS). Acute myocardial ischaemia was induced by transient coronary occlusion during percutaneous coronary intervention (PCI). Plasma samples were collected at baseline (prior to PCI) and at 1 and 5 min post-coronary occlusion. Samples were analyzed by Ultra Performance Liquid Chromatography-Mass Spectrometry in an untargeted metabolomics approach.
Results
The study observed changes in the circulating levels of metabolites at 1 and 5 min following transient coronary ischaemia. Both DS and VS identified 54 and 55 metabolites as significant (P < 0.05) when compared to baseline levels, respectively. Fatty acid beta-oxidation and anaerobic respiration, lysoglycerophospholipids, arachidonic acid, docosahexaenoic acid, tryptophan metabolism and sphingosine-1-phosphate were identified as mechanistically important.
Conclusion
Using an untargeted metabolomics approach, the study identified important cardiac metabolic changes in peripheral and coronary sinus plasma, in a human model of controlled acute myocardial ischaemia. Distinct classes of metabolites were shown to be involved in the rapid cardiac response to ischemia and provide insights into diagnostic and interventional targets.</description><identifier>ISSN: 1573-3882</identifier><identifier>EISSN: 1573-3890</identifier><identifier>DOI: 10.1007/s11306-021-01827-x</identifier><identifier>PMID: 34424431</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Anaerobic respiration ; Arachidonic acid ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Coronary Artery Disease ; Coronary Occlusion ; Developmental Biology ; Docosahexaenoic acid ; Heart ; Humans ; Ischemia ; Life Sciences ; Liquid chromatography ; Mass spectroscopy ; Metabolism ; Metabolites ; Metabolome ; Metabolomics ; Molecular Medicine ; Occlusion ; Original ; Original Article ; Oxidation ; Percutaneous Coronary Intervention ; Sphingosine 1-phosphate ; Tryptophan</subject><ispartof>Metabolomics, 2021-09, Vol.17 (9), p.76-76, Article 76</ispartof><rights>Crown 2021</rights><rights>2021. Crown.</rights><rights>Crown 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a9b33bd7196f62601435bf20f3bebe19ec34ea27fb3b434cf3aa12da4de135ad3</citedby><cites>FETCH-LOGICAL-c474t-a9b33bd7196f62601435bf20f3bebe19ec34ea27fb3b434cf3aa12da4de135ad3</cites><orcidid>0000-0003-1699-6247</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34424431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chacko, Sanoj</creatorcontrib><creatorcontrib>Mamas, Mamas A.</creatorcontrib><creatorcontrib>El-Omar, Magdi</creatorcontrib><creatorcontrib>Simon, David</creatorcontrib><creatorcontrib>Haseeb, Sohaib</creatorcontrib><creatorcontrib>Fath-ordoubadi, Farzin</creatorcontrib><creatorcontrib>Clarke, Bernard</creatorcontrib><creatorcontrib>Neyses, Ludwig</creatorcontrib><creatorcontrib>Dunn, Warwick B.</creatorcontrib><title>Perturbations in cardiac metabolism in a human model of acute myocardial ischaemia</title><title>Metabolomics</title><addtitle>Metabolomics</addtitle><addtitle>Metabolomics</addtitle><description>Introduction
Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to define these metabolic perturbations on a metabolome-wide scale.
Objectives
This study was designed to identify cardiac metabolic changes in serum during the first 5 min following early myocardial ischaemia in humans, applying an untargeted metabolomics approach.
Methods
Peripheral venous samples were collected from 46 patients in a discovery study (DS) and a validation study (VS) (25 for DS, 21 for VS). Coronary sinus venous samples were collected from 7 patients (4 for DS, 3 for VS). Acute myocardial ischaemia was induced by transient coronary occlusion during percutaneous coronary intervention (PCI). Plasma samples were collected at baseline (prior to PCI) and at 1 and 5 min post-coronary occlusion. Samples were analyzed by Ultra Performance Liquid Chromatography-Mass Spectrometry in an untargeted metabolomics approach.
Results
The study observed changes in the circulating levels of metabolites at 1 and 5 min following transient coronary ischaemia. Both DS and VS identified 54 and 55 metabolites as significant (P < 0.05) when compared to baseline levels, respectively. Fatty acid beta-oxidation and anaerobic respiration, lysoglycerophospholipids, arachidonic acid, docosahexaenoic acid, tryptophan metabolism and sphingosine-1-phosphate were identified as mechanistically important.
Conclusion
Using an untargeted metabolomics approach, the study identified important cardiac metabolic changes in peripheral and coronary sinus plasma, in a human model of controlled acute myocardial ischaemia. Distinct classes of metabolites were shown to be involved in the rapid cardiac response to ischemia and provide insights into diagnostic and interventional targets.</description><subject>Anaerobic respiration</subject><subject>Arachidonic acid</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Coronary Artery Disease</subject><subject>Coronary Occlusion</subject><subject>Developmental Biology</subject><subject>Docosahexaenoic acid</subject><subject>Heart</subject><subject>Humans</subject><subject>Ischemia</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Metabolomics</subject><subject>Molecular Medicine</subject><subject>Occlusion</subject><subject>Original</subject><subject>Original Article</subject><subject>Oxidation</subject><subject>Percutaneous Coronary Intervention</subject><subject>Sphingosine 1-phosphate</subject><subject>Tryptophan</subject><issn>1573-3882</issn><issn>1573-3890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kU9rFjEQxoMotla_gAcJePGymmSyye5FkOI_KCilnsMkm-2bstnUZFfab2_ara-1B08TZn7zTB4eQl5y9pYzpt8VzoGphgneMN4J3Vw9Ioe81dBA17PH-3cnDsizUi4Yk7LX7Ck5ACmFlMAPyel3n5c1W1xCmgsNM3WYh4CORr-gTVMo8aaLdLdGnGlMg59oGim6dfE0XqeNn2goboc-BnxOnow4Ff_irh6RH58-nh1_aU6-ff56_OGkcVLLpcHeAthB816NSijGJbR2FGwE663nvXcgPQo9WrASpBsBkYsB5eA5tDjAEXm_6V6uNvrB-XnJOJnLHCLma5MwmH8nc9iZ8_TLdNAJJfsq8OZOIKefqy-LidWEnyacfVqLEa0CzVULrKKvH6AXac1ztXdLtUorgEqJjXI5lZL9uP8MZ-YmMrNFZmpk5jYyc1WXXt23sV_5k1EFYANKHc3nPv-9_R_Z31PGo7c</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Chacko, Sanoj</creator><creator>Mamas, Mamas A.</creator><creator>El-Omar, Magdi</creator><creator>Simon, David</creator><creator>Haseeb, Sohaib</creator><creator>Fath-ordoubadi, Farzin</creator><creator>Clarke, Bernard</creator><creator>Neyses, Ludwig</creator><creator>Dunn, Warwick B.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1699-6247</orcidid></search><sort><creationdate>20210901</creationdate><title>Perturbations in cardiac metabolism in a human model of acute myocardial ischaemia</title><author>Chacko, Sanoj ; Mamas, Mamas A. ; El-Omar, Magdi ; Simon, David ; Haseeb, Sohaib ; Fath-ordoubadi, Farzin ; Clarke, Bernard ; Neyses, Ludwig ; Dunn, Warwick B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-a9b33bd7196f62601435bf20f3bebe19ec34ea27fb3b434cf3aa12da4de135ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anaerobic respiration</topic><topic>Arachidonic acid</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Coronary Artery Disease</topic><topic>Coronary Occlusion</topic><topic>Developmental Biology</topic><topic>Docosahexaenoic acid</topic><topic>Heart</topic><topic>Humans</topic><topic>Ischemia</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolome</topic><topic>Metabolomics</topic><topic>Molecular Medicine</topic><topic>Occlusion</topic><topic>Original</topic><topic>Original Article</topic><topic>Oxidation</topic><topic>Percutaneous Coronary Intervention</topic><topic>Sphingosine 1-phosphate</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chacko, Sanoj</creatorcontrib><creatorcontrib>Mamas, Mamas A.</creatorcontrib><creatorcontrib>El-Omar, Magdi</creatorcontrib><creatorcontrib>Simon, David</creatorcontrib><creatorcontrib>Haseeb, Sohaib</creatorcontrib><creatorcontrib>Fath-ordoubadi, Farzin</creatorcontrib><creatorcontrib>Clarke, Bernard</creatorcontrib><creatorcontrib>Neyses, Ludwig</creatorcontrib><creatorcontrib>Dunn, Warwick B.</creatorcontrib><collection>SpringerOpen (Open Access)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Metabolomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chacko, Sanoj</au><au>Mamas, Mamas A.</au><au>El-Omar, Magdi</au><au>Simon, David</au><au>Haseeb, Sohaib</au><au>Fath-ordoubadi, Farzin</au><au>Clarke, Bernard</au><au>Neyses, Ludwig</au><au>Dunn, Warwick B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perturbations in cardiac metabolism in a human model of acute myocardial ischaemia</atitle><jtitle>Metabolomics</jtitle><stitle>Metabolomics</stitle><addtitle>Metabolomics</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>17</volume><issue>9</issue><spage>76</spage><epage>76</epage><pages>76-76</pages><artnum>76</artnum><issn>1573-3882</issn><eissn>1573-3890</eissn><abstract>Introduction
Acute myocardial ischaemia and the transition from reversible to irreversible myocardial injury are associated with abnormal metabolic patterns. Advances in metabolomics have extended our capabilities to define these metabolic perturbations on a metabolome-wide scale.
Objectives
This study was designed to identify cardiac metabolic changes in serum during the first 5 min following early myocardial ischaemia in humans, applying an untargeted metabolomics approach.
Methods
Peripheral venous samples were collected from 46 patients in a discovery study (DS) and a validation study (VS) (25 for DS, 21 for VS). Coronary sinus venous samples were collected from 7 patients (4 for DS, 3 for VS). Acute myocardial ischaemia was induced by transient coronary occlusion during percutaneous coronary intervention (PCI). Plasma samples were collected at baseline (prior to PCI) and at 1 and 5 min post-coronary occlusion. Samples were analyzed by Ultra Performance Liquid Chromatography-Mass Spectrometry in an untargeted metabolomics approach.
Results
The study observed changes in the circulating levels of metabolites at 1 and 5 min following transient coronary ischaemia. Both DS and VS identified 54 and 55 metabolites as significant (P < 0.05) when compared to baseline levels, respectively. Fatty acid beta-oxidation and anaerobic respiration, lysoglycerophospholipids, arachidonic acid, docosahexaenoic acid, tryptophan metabolism and sphingosine-1-phosphate were identified as mechanistically important.
Conclusion
Using an untargeted metabolomics approach, the study identified important cardiac metabolic changes in peripheral and coronary sinus plasma, in a human model of controlled acute myocardial ischaemia. Distinct classes of metabolites were shown to be involved in the rapid cardiac response to ischemia and provide insights into diagnostic and interventional targets.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34424431</pmid><doi>10.1007/s11306-021-01827-x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1699-6247</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anaerobic respiration Arachidonic acid Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Coronary Artery Disease Coronary Occlusion Developmental Biology Docosahexaenoic acid Heart Humans Ischemia Life Sciences Liquid chromatography Mass spectroscopy Metabolism Metabolites Metabolome Metabolomics Molecular Medicine Occlusion Original Original Article Oxidation Percutaneous Coronary Intervention Sphingosine 1-phosphate Tryptophan |
| title | Perturbations in cardiac metabolism in a human model of acute myocardial ischaemia |
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