Repair of brain damage size and recovery of neurological dysfunction after ischemic stroke are different between strains in mice: evaluation using a novel ischemic stroke model

• Published in: Experimental Animals 2021, Vol.70(3), pp.344-354
• Main Authors: Matano, Yasuki, Nojiri, Yuuto, Nomura, Mizuki, Masuda, Akira, Moriike, Yuuki, Suzuki, Yasuhiro, Umemura, Kazuo, Nagai, Nobuo
• Format: Article
• Language: English
• Subjects: Abnormalities; Brain; Brain damage; Brain injury; Cortex (frontal); Damage accumulation; Damage assessment; Edema; Frontal lobe; Ischemia; ischemic stroke; microglia; Neurological complications; neurological dysfunction; Original; Photochemical reactions; Photochemicals; Recovery; Repair; strains; Stroke
• ISSN: 1341-1357; 1881-7122
• DOI: 10.1538/expanim.20-0182

In the current study, we established a novel murine ischemic brain damage model using a photochemical reaction to evaluate the recovery of neurological dysfunction and brain repair reactions. In this model, reproducible damage was induced in the frontal lobe of the cortex, which was accompanied by neurological dysfunction. Sequential changes in damage size, microglial accumulation, astrocyte activation, and neurological dysfunction were studied in C57BL/6J and BALB/c mouse strains. Although the initial size of damage was comparable in both strains, the extent of damage was later reduced to a greater extent in C57BL/6J mice than that in BALB/c mice. In addition, C57BL/6J mice showed later edema clearance until day 7, less microglial accumulation, and relatively more astrocyte activation on day 7. Neurologic dysfunction was evaluated by three behavioral tests: the von Frey test, the balance beam test, and the tail suspension test. The behavioral abnormalities evaluated by these tests were remarkable following the induction of damage and recovered by day 21 in both strains. However, the abnormalities were more prominent and the recovery was later in C57BL/6J mice. These findings demonstrate that our novel ischemic stroke model is useful for evaluating brain repair reactions and the recovery of neurological dysfunction in mice with different genetic backgrounds. In addition, we found that both the brain repair reactions and the recovery of neurological dysfunction after comparable ischemic brain damage varied between strains; in that, they both occurred later in C57BL/6J mice.