Biochemical Characterization of a Novel Exo-Type PL7 Alginate Lyase VsAly7D from Marine Vibrio sp. QY108

Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L- -5-hexoseulose uronate (DEH) by a non-enz...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (16), p.8402
Main Authors: Zhang, Fengchao, Fu, Zheng, Tang, Luyao, Zhang, Zhelun, Han, Feng, Yu, Wengong
Format: Article
Language:English
Subjects:
Algae
Alginate lyase
Alginates
Alginates - metabolism
Alginic acid
Amino Acid Sequence
Amino acids
Bacteria
Bacterial Proteins - metabolism
Biodiesel fuels
Biofuels
Carbohydrates
Cell walls
Cold
E coli
Enzymes
Escherichia coli - metabolism
Ethanol
Glucuronic Acid - metabolism
Hydrogen-Ion Concentration
Industrial production
Molecular weight
Monosaccharides
Monosaccharides - metabolism
Phaeophyceae
Phaeophyceae - microbiology
Phylogenetics
Polysaccharide-Lyases - metabolism
Proteins
Renewable resources
Vibrio
Vibrio - metabolism
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Summary:Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L- -5-hexoseulose uronate (DEH) by a non-enzyme reaction, which is an important raw material for the preparation of bioethanol. In our research, a novel exo-type alginate lyase, VsAly7D, belonging to the PL7 family was isolated from marine bacterium sp. QY108 and recombinantly expressed in . The purified VsAly7D demonstrated the highest activity at 35 °C, whereas it still maintained 46.5% and 83.1% of its initial activity at 20 °C and 30 °C, respectively. In addition, VsAly7D exhibited the maximum activity under alkaline conditions (pH 8.0), with the simultaneously remaining stability between pH 8.0 and 10.0. Compared with other reported exo-type enzymes, VsAly7D could efficiently degrade alginate, poly-β-D-mannuronate (polyM) and poly-α-L-guluronate (polyG) with highest specific activities (663.0 U/mg, 913.6 U/mg and 894.4 U/mg, respectively). These results showed that recombinant VsAly7D is a suitable tool enzyme for unsaturated alginate monosaccharide preparation and holds great promise for producing bioethanol from brown algae.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22168402