DNA Damage, n-3 Long-Chain PUFA Levels and Proteomic Profile in Brazilian Children and Adolescents

Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, fo...

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Bibliographic Details
Published in:Nutrients 2021-07, Vol.13 (8), p.2483
Main Authors: Barros, Tamiris Trevisan de, Venancio, Vinicius de Paula, Hernandes, Lívia Cristina, Antunes, Lusania Maria Greggi, Hillesheim, Elaine, Salomão, Roberta Garcia, Mathias, Mariana Giaretta, Coelho-Landell, Carolina Almeida, Toffano, Roseli Borges Donegá, Almada, Maria Olimpia Ribeiro do Vale, Camelo-Junior, José Simon, Moco, Sofia, Cominetti, Ornella, Ued, Fábio da Veiga, Kaput, Jim, Monteiro, Jacqueline Pontes
Format: Article
Language:English
Subjects:
Adolescents
Blood levels
Body mass index
Cell cycle
Children
Comet assay
Cyclin-dependent kinases
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
Docosahexaenoic acid
Eicosapentaenoic acid
Ethylenediaminetetraacetic acid
Fatty acids
Gene expression
Inflammation
Nutrition
Omega-3 fatty acids
Physiology
Proteins
Proteomics
Teenagers
Toxicity
Unsaturated fatty acids
Urban areas
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Summary:Fatty acids play a significant role in maintaining cellular and DNA protection and we previously found an inverse relationship between blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and DNA damage. The aim of this study was to explore differences in proteomic profiles, for 117 pro-inflammatory proteins, in two previously defined groups of individuals with different DNA damage and EPA and DHA levels. Healthy children and adolescents (n = 140) aged 9 to 13 years old in an urban area of Brazil were divided by k-means cluster test into two clusters of DNA damage (tail intensity) using the comet assay (cluster 1 = 5.9% ± 1.2 and cluster 2 = 13.8% ± 3.1) in our previous study. The cluster with higher DNA damage and lower levels of DHA (6.2 ± 1.6 mg/dL; 5.4 ± 1.3 mg/dL, p = 0.003) and EPA (0.6 ± 0.2 mg/dL; 0.5 ± 0.1 mg/dL, p < 0.001) presented increased expression of the proteins CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB, which are involved in pro-inflammatory pathways. Our findings support the hypothesis that low levels of n-3 long-chain PUFA may have a less protective role against DNA damage through expression of pro-inflammatory proteins, such as CDK8–CCNC, PIK3CA–PIK3R1, KYNU, and PRKCB.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu13082483