Optimization of food-grade medium for co-production of bioactive substances by Lactobacillus acidophilus LA-5 for explaining pharmabiotic mechanisms of probiotic

• Published in: Journal of food science and technology 2021-11, Vol.58 (11), p.1-12
• Main Authors: Amiri, Saber, Mokarram, Reza Rezaei, Khiabani, Mahmoud Sowti, Bari, Mahmoud Rezazadeh, Alizadeh, Mohammad
• Format: Article
• Language: English
• Subjects: Bacteriocins; Bioactive compounds; Biological activity; Cell number; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Dairy products; Exopolysaccharides; Factorial design; Food; Food Science; Functional groups; Galactose; Isomers; Lactobacilli; Lactobacillus acidophilus; Linoleic acid; Mannose; Metabolites; Molecular weight; Nutrition; Optimization; Original; Original Article; Probiotics; Response surface methodology; Whey; Yeasts
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-020-04894-5

This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA-5 in dairy food-grade by-product. The factorial design revealed that the significant factors were temperature, time, and yeast extract. Then the response surface methodology was used for optimization. At the optimal conditions the viable cell number, CLA, EPSs, and inhibition activity were 2.62 ± 0.49 × 10 8  CFU/mL, 51.46 ± 1.50 μg/mL, 348.24 ± 5.61 mg/mL and 12.46 ± 0.80 mm, respectively. FTIR, GC, TLC, and SDS page analysis revealed the functional groups of pharmabiotics. The FTIR, GC, TLC, and SDS page analysis showed that both CLA isomers ( c-9 , t-11 , and t-10 , c-12 ) produced. The FTIR, GC, TLC, and SDS page analysis indicated that produced EPSs were composed of glucose, mannose, galactose, xylose, and fructose. FTIR, GC, TLC, and SDS page used to report BACs molecular weight, which showed two fractions by molecular mass 35 and 63 kDa. Previously the ability of different probiotic bacteria investigated and optimized the production of CLA, EPSs, and BACs, but, there was no report on the co-producing capacity of these bioactive metabolites by probiotics. The present work was investigated to optimize the co-production of pharmabiotic metabolites by L. acidophilus LA-5, in supplemented cheese whey as a cultivation medium.