Rapid identification of Candida glabrata by using a dipstick to detect trehalase-generated glucose

Candida glabrata is a yeast frequently isolated from human specimens. Based upon its well-known ability to rapidly hydrolyze trehalose, we have developed a novel and cost-effective test incubating one yeast colony emulsified in 50 microl of citrate buffer (0.1 M [pH 5. 0]) containing 4% (wt/vol) tre...

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Bibliographic Details
Published in:Journal of clinical microbiology 1999, Vol.37 (1), p.202-205
Main Authors: PELTROCHE-LLACSAHUANGA, H, SCHNITZLER, N, LÜTTICKEN, R, HAASE, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Candida - classification
Candida - enzymology
Candida - isolation & purification
Candida - metabolism
Candida glabrata
Candidiasis - diagnosis
Candidiasis - microbiology
Fundamental and applied biological sciences. Psychology
Fungemia - diagnosis
Fungemia - microbiology
Glucose - analysis
Humans
Microbiological Techniques
Microbiology
Mycological methods and techniques used in mycology
Mycology
Reagent Kits, Diagnostic
Sensitivity and Specificity
Trehalase - metabolism
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Summary:Candida glabrata is a yeast frequently isolated from human specimens. Based upon its well-known ability to rapidly hydrolyze trehalose, we have developed a novel and cost-effective test incubating one yeast colony emulsified in 50 microl of citrate buffer (0.1 M [pH 5. 0]) containing 4% (wt/vol) trehalose for 3 h at 37 degrees C. Trehalase-generated glucose is detected with a commercially available dipstick (range, 1.0 to 50 g/liter). For evaluation, consecutive clinical isolates and several reference strains of C. glabrata (n = 160), C. albicans (n = 120), and other yeast species with potential ability for utilization of trehalose (C. dubliniensis, n = 11; C. famata, n = 15; C. guilliermondii, n = 5; C. lusitaniae, n = 16; C. parapsilosis, n = 20; C. tropicalis, n = 34; C. viswanathii, n = 5; Pichia angusta, n = 2; C. zeylanoides, n = 2; Saccharomyces cerevisiae, n = 16; C. neoformans, n = 7) were tested. Identification of C. glabrata is achieved within 3 h, with a specificity of 99.1% and a sensitivity of 98.8% when grown on Sabouraud dextrose agar supplemented with 4% glucose.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.37.1.202-205.1999