Protein Adsorption onto Modified Porous Silica by Single and Binary Human Serum Protein Solutions

Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were character...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (17), p.9164
Main Authors: Gondim, Diego R, Cecilia, Juan A, Rodrigues, Thaina N B, Vilarrasa-García, Enrique, Rodríguez-Castellón, Enrique, Azevedo, Diana C S, Silva, Jr, Ivanildo J
Format: Article
Language:English
Subjects:
Adsorbents
Adsorption
Antibodies
Chemical bonds
Fluorides
Human serum albumin
Humans
IgG antibody
Immunoglobulin G
Immunoglobulins
Nanoparticles
Photoelectron spectroscopy
Photoelectrons
Polypeptides
Porosity
Porous materials
Protein adsorption
Proteins
Serum albumin
Serum Albumin - chemistry
Serum Globulins - chemistry
Serum proteins
Silica
Silicon Dioxide - chemistry
Trimethylbenzene
X ray photoelectron spectroscopy
X-ray diffraction
Zirconium
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Summary:Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N adsorption/desorption isotherms at -196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420-337 m g and an average pore diameter with a maximum between 20-25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin-HSA and immunoglobulin G-IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22179164