Investigation on Green Synthesis, Biocompatibility, and Antibacterial Activity of Silver Nanoparticles Prepared Using Cistus incanus

This paper describes the plant-mediated preparation of silver nanoparticles with aqueous extract and infusion of Cistus incanus leaves. To evaluate aqueous extract and infusion antioxidant capacity and total phenolic content the DPPH and Folin–Ciocalteau methods were utilized. The antioxidant capaci...

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Bibliographic Details
Published in:Materials 2021-09, Vol.14 (17), p.5028
Main Authors: Florkiewicz, Wioletta, Pluta, Klaudia, Malina, Dagmara, Rudnicka, Karolina, Żywicka, Anna, Guigou, Martin Duarte, Tyliszczak, Bożena, Sobczak-Kupiec, Agnieszka
Format: Article
Language:English
Subjects:
Absorption spectra
Acids
Antioxidants
Biocompatibility
Biosynthesis
Calibration
Equivalence
Ethanol
Fibroblasts
Flavonoids
Gallic acid
Medical research
Metabolites
Morphology
Nanomaterials
Nanoparticles
Natural products
Phase composition
Phytochemicals
Silver
Toxicity
Weight
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Summary:This paper describes the plant-mediated preparation of silver nanoparticles with aqueous extract and infusion of Cistus incanus leaves. To evaluate aqueous extract and infusion antioxidant capacity and total phenolic content the DPPH and Folin–Ciocalteau methods were utilized. The antioxidant capacity and total phenolic content of extract and infusion were equal to 85.97 ± 6.54 mg gallic acid equivalents per gram of dry weight.; 10.76 ± 0.59 mg/mL and 12.65 ± 1.04 mg gallic acid equivalents per gram of dry weight.; 3.10 ± 0.14 mg/mL, respectively. The formed nanoparticles displayed the characteristic absorption band in the 380–450 nm wavelength range. The average size of particles was in the 68.8–71.2 nm range. Morphology and phase composition analysis revealed the formation of spherical nanoparticles with a face-centred cubic structure. Immune compatibility tests of nanoparticles and plant extracts showed no activation of the THP1-XBlue™ monocyte. Cytotoxicity tests performed with L929 mice fibroblasts showed that nanoparticles should be utilized at a concentration of 16 ppm. The minimum inhibitory concentrations determined with the microdilution method for nanoparticles prepared with plant infusion for S. aureus and S. epidermidis were 2 ppm and 16 ppm, respectively.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma14175028