Discovery of potential biomarkers for human atherosclerotic abdominal aortic aneurysm through untargeted metabolomics and transcriptomics

Abdominal aortic aneurysm (AAA) and atherosclerosis (AS) have considerable similarities in clinical risk factors and molecular pathogenesis. The aim of our study was to investigate the differences between AAA and AS from the perspective of metabolomics, and to explore the potential mechanisms of dif...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2021-09, Vol.22 (9), p.733-745
Main Authors: Ji, Lei, Chen, Siliang, Gu, Guangchao, Wang, Wei, Ren, Jinrui, Xu, Fang, Li, Fangda, Wu, Jianqiang, Yang, Dan, Zheng, Yuehong
Format: Article
Language:English
Subjects:
Aged
Aorta
Aortic Aneurysm, Abdominal - metabolism
Aortic aneurysms
Arteriosclerosis
Atherosclerosis
Atherosclerosis - metabolism
Biomarkers
Biomedical and Life Sciences
Biomedicine
D-Ribose
Female
Gene Expression Profiling - methods
Humans
Liquid chromatography
Male
Mass spectrometry
Mass spectroscopy
Metabolites
Metabolomics
Metabolomics - methods
Middle Aged
Pathogenesis
Patients
Research Article
Ribose
Risk analysis
Risk factors
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Summary:Abdominal aortic aneurysm (AAA) and atherosclerosis (AS) have considerable similarities in clinical risk factors and molecular pathogenesis. The aim of our study was to investigate the differences between AAA and AS from the perspective of metabolomics, and to explore the potential mechanisms of differential metabolites via integration analysis with transcriptomics. Plasma samples from 32 AAA and 32 AS patients were applied to characterize the metabolite profiles using untargeted liquid chromatography-mass spectrometry (LC-MS). A total of 18 remarkably different metabolites were identified, and a combination of seven metabolites could potentially serve as a biomarker to distinguish AAA and AS, with an area under the curve (AUC) of 0.93. Subsequently, we analyzed both the metabolomics and transcriptomics data and found that seven metabolites, especially 2′-deoxy- d -ribose (2dDR), were significantly correlated with differentially expressed genes. In conclusion, our study presents a comprehensive landscape of plasma metabolites in AAA and AS patients, and provides a research direction for pathogenetic mechanisms in atherosclerotic AAA.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B2000713