Immunodiagnostic usefulness of monoclonal antibodies specific to conformational epitopes of Taenia solium oncosphere protein TSOL18

• Published in: Journal of immunological methods 2021-10, Vol.497, p.113121-113121, Article 113121
• Main Authors: Assana, Emmanuel, Zoli, André Pagnah, Gauci, Charles G., Lightowlers, Marshall W., Dorny, Pierre
• Format: Article
• Language: English
• Subjects: Epitopes; Immunodiagnosis; Monoclonal antibody; Taenia solium; TSOL18
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2021.113121

Taenia solium oncosphere protein TSOL18 is the host-protective antigen against porcine cysticercosis. Little attention has been given to use it as target molecule in immunodiagnostic tests. The objective of this paper is to describe the immunodiagnostic potential of monoclonal antibodies (MoAbs) raised against conformational epitopes of TSOL18. Three murine IgG1 MoAbs (25D12C1, 21C2D2, 10H1F2) against three different conformational epitopes of TSOL18 were produced and evaluated with an inhibition enzyme-linked immunosorbent assay (i-ELISA) for the detection of anti-TSOL18 and anti-oncosphere antibodies. Serum samples from pigs immunized with TSOL18 inhibited the binding of the three MoAbs to TSOL18 antigen in i-ELISA. The highest inhibition of anti-TSOL18 antibodies in immunized pigs was observed with MoAb 25D12C1. Ten field sera (12.19%) from 82 non-vaccinated and non-infected pigs showed anti-oncosphere antibodies inhibiting the binding of MoAb 25D12C1. Anti-oncosphere antibodies in pigs experimentally infected with T. solium eggs inhibited the binding of MoAb 25D12C1 from 2 to 8 week-post infection. It is concluded that MoAb 25D12C1 has excellent immunodiagnostic potentials to detect anti-oncosphere antibodies in the intermediate hosts at early exposure to T. solium eggs. Further investigations on potential use of MoAb 25D12C1 in a capture antigen ELISA for the detection of post-oncospheral antigens in infected pigs cannot be overemphasized. [Display omitted] •We describe the potential usefulness of the monoclonal antibody 25D12C1 to TSOL18 protein.•Serum samples from pigs immunized with TSOL18 inhibited the binding of 25D12C1 in an inhibition enzyme-linked immunosorbent assay (i-ELISA) for the detection of anti-TSOL18 and anti-oncosphere antibodies.•Anti-oncosphere antibodies in pigs infected with T. solium eggs inhibited also the binding of 25D12C1.•It is concluded that MoAb i-ELISA using 25D12C1 has the potential to identify animals exposed to T. solium infection.