Bile Salts Regulate Zinc Uptake and Capsule Synthesis in a Mastitis-Associated Extraintestinal Pathogenic Escherichia coli Strain

• Published in: Infection and immunity 2021-09, Vol.89 (10), p.e0035721-e0035721
• Main Authors: Olson, Michael A, Grimsrud, Aleksander, Richards, Amanda C, Mulvey, Matthew A, Wilson, Eric, Erickson, David L
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Capsules - metabolism; Bile Acids and Salts - metabolism; Escherichia coli Infections - metabolism; Escherichia coli Infections - microbiology; Escherichia coli Proteins - metabolism; Extraintestinal Pathogenic Escherichia coli - metabolism; Female; Host-Microbial Interactions; Male; Mastitis - metabolism; Mastitis - microbiology; Mice; Mice, Inbred C57BL; Molecular Pathogenesis; Sepsis - metabolism; Sepsis - microbiology; Virulence - physiology; Virulence Factors - metabolism; Zinc - metabolism
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00357-21

Extraintestinal pathogenic Escherichia coli (ExPEC) strains are major causes of urinary and bloodstream infections. ExPEC reservoirs are not completely understood. Some mastitis-associated E. coli (MAEC) strains carry genes associated with ExPEC virulence, including metal scavenging, immune avoidance, and host attachment functions. In this study, we investigated the role of the high-affinity zinc uptake ( ) system in the MAEC strain M12. Elimination of moderately decreased fitness during mouse mammary gland infections. The Δ mutant strain exhibited an unexpected growth delay in the presence of bile salts, which was alleviated by the addition of excess zinc. We isolated suppressor mutants with improved growth in bile salts, several of which no longer produced the K96 capsule made by strain M12. The addition of bile salts also reduced capsule production by strain M12 and ExPEC strain CP9, suggesting that capsule synthesis may be detrimental when bile salts are present. To better understand the role of the capsule, we compared the virulence of mastitis strain M12 with that of its unencapsulated Δ mutant in two models of ExPEC disease. The wild-type strain successfully colonized mouse bladders and kidneys and was highly virulent in intraperitoneal infections. Conversely, the Δ mutant was unable to colonize kidneys and was unable to cause sepsis. These results demonstrate that some MAEC strains may be capable of causing human ExPEC illness. The virulence of strain M12 in these infections is dependent on its capsule. However, capsule may interfere with zinc homeostasis in the presence of bile salts while in the digestive tract.